Detailed information of TA system TA158808

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3721429..3722088
• Replicon: chromosome
• Accession: NZ_CP054160
• Organism: Serratia fonticola strain HH13

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: G9399_RS17115
• Protein ID: WP_173409016.1
• Coordinates: 3721735..3722088 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: U2NFA4
• Locus tag: G9399_RS17110
• Protein ID: WP_021180280.1
• Coordinates: 3721429..3721731 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9399_RS17090 (G9399_09375)	3716756..3717925	+	1170	WP_024528107.1	flavin-dependent monooxygenase	-
G9399_RS17095 (G9399_09370)	3717946..3718890	+	945	WP_065683277.1	flavin reductase family protein	-
G9399_RS17100 (G9399_09365)	3718963..3720165	-	1203	WP_173409018.1	Bcr/CflA family multidrug efflux MFS transporter	-
G9399_RS17105 (G9399_09360)	3720425..3721285	-	861	WP_173409017.1	aldo/keto reductase family oxidoreductase	-
G9399_RS17110 (G9399_09355)	3721429..3721731	-	303	WP_021180280.1	XRE family transcriptional regulator	Antitoxin
G9399_RS17115 (G9399_09350)	3721735..3722088	-	354	WP_173409016.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G9399_RS17120 (G9399_09345)	3722346..3723533	-	1188	WP_024528102.1	sugar transporter	-
G9399_RS17125 (G9399_09340)	3723705..3724421	-	717	WP_065683272.1	ABC transporter ATP-binding protein	-
G9399_RS17130 (G9399_09335)	3724418..3725293	-	876	WP_024528101.1	ATP-binding cassette domain-containing protein	-
G9399_RS17135 (G9399_09330)	3725290..3726576	-	1287	WP_024528100.1	high-affinity branched-chain amino acid ABC transporter permease LivM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13606.61 Da
• Isoelectric Point: 9.9193

>T158808 WP_173409016.1 NZ_CP054160:c3722088-3721735 [Serratia fonticola]
VWEIKTTDMFDEWFSRLEDTDRASVLASLIVLREKGPQLPRPYADTVKGSRYSNMKELRVQSRGIPIRAFFAFDLRRRGI
LLCAGNKAGNEKRFYEVMIPIADRAFTQFLNTSENKE

Nucleotide

• Length: 354 bp

>T158808 NZ_CP069847:2113886-2114104 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11022.78 Da
• Isoelectric Point: 6.4749

>AT158808 WP_021180280.1 NZ_CP054160:c3721731-3721429 [Serratia fonticola]
MGRTLEQILAEEKPEVVASAERIAADTLLNIHLAELRERVQKTQVEMAQALGIKQPTVAGMEKPGRDLKLSSLKRYIEAA
GGKLRLDIELPDGSHYGFVI

Nucleotide

• Length: 303 bp

>AT158808 NZ_CP069847:2113485-2113859 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: U2NFA4