Detailed information of TA system TA158766

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 149027..149612
• Replicon: plasmid pHNJXA13-1
• Accession: NZ_CP054138
• Organism: Acinetobacter pittii strain JXA13

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: HRJ47_RS20030
• Protein ID: WP_000897307.1
• Coordinates: 149292..149612 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HRJ47_RS20025
• Protein ID: WP_000369781.1
• Coordinates: 149027..149299 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRJ47_RS20010	145318..145929	+	612	WP_015060246.1	recombinase family protein	-
HRJ47_RS20015	146119..147003	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
HRJ47_RS20020	147059..148534	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
HRJ47_RS20025	149027..149299	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
HRJ47_RS20030	149292..149612	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HRJ47_RS20035	149752..150171	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
HRJ47_RS20040	150251..150532	-	282	WP_000985609.1	putative addiction module antidote protein	-
HRJ47_RS20045	150533..150835	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HRJ47_RS20050	150928..151407	-	480	WP_065995571.1	cysteine hydrolase family protein	-
HRJ47_RS20055	151489..152579	+	1091	WP_100222571.1	IS4-like element ISAba1 family transposase	-
HRJ47_RS20060	153092..154426	+	1335	WP_203080106.1	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(X3) / sul2 / blaNDM-1 / aph(3')-VIa / aac(3)-IId / tet(39) / mph(E) / msr(E)	-	1..206931	206931
	-	inside	IScluster/Tn	aac(3)-IId / tet(39) / mph(E) / msr(E)	-	132850..154426	21576

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T158766 WP_000897307.1 NZ_CP054138:c149612-149292 [Acinetobacter pittii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T158766 NZ_CP069811:1780080-1780358 [Cupriavidus oxalaticus]
ATGATCCAATCGTTCAGATGCTTTGACACCGCTGCGTTGTTCGACGGCCGTTACGTTGCCCGCTTCGCGAGCATTGAGTT
CATTGCCCAGCGCAAGCTCGAGATGCTTGACGACGCCGCCACCCTGGGCGCGTTGCGCTTTCCGCCGGGAAACCGCCTGG
AAAAACTCGGCGGCGATCGCAAGGGACGCTACAGCATCCGCATCAACGGCCAGTGGAGGATCTGCTTCATCTGGACCCAT
GACGGCCCCGCGGCTGTCGAGATCATCGACTATCACTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT158766 WP_000369781.1 NZ_CP054138:c149299-149027 [Acinetobacter pittii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT158766 NZ_CP069811:1780369-1780698 [Cupriavidus oxalaticus]
ATGGCACGCTTTGAAAACGGCATGCGCCCCATCCATCCGGGCGAAATCCTGCGCGAGGAATACCTGGTGCCGCTGAACAT
GAGCGCCAACGCCCTGGCGCTGGCACTGCGCGTGACGCCGGCCCGCATCAGCGAGATCGTGCGCGAGCAGCGCGGCATCA
CGCCCGACACCGCGCTGCGCCTGACGCGCTATTTTGGTGGCGATGCGCGTTCGTGGATGAATATGCAGGTGACCTACGAC
CTCAAGGTGGCGCAGCGGGACCTGGGCGAGCGCATCGCGGCGGAAGTGGTGCCGCGCGCGGGTATCGACCAGGAAATTCC
GGAAAGCTAG

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3