Detailed information of TA system TA158761

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4772322..4773076
• Replicon: chromosome
• Accession: NZ_CP054129
• Organism: Citrobacter portucalensis strain BY9F36

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5B0T5Y1
• Locus tag: HRV88_RS22725
• Protein ID: WP_048215202.1
• Coordinates: 4772322..4772633 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HRV88_RS22730
• Protein ID: WP_235125977.1
• Coordinates: 4772630..4773076 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRV88_RS22695 (HRV88_22630)	4767984..4768886	+	903	WP_003825273.1	formate dehydrogenase O subunit beta	-
HRV88_RS22700 (HRV88_22635)	4768883..4769518	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
HRV88_RS22705 (HRV88_22640)	4769515..4770444	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
HRV88_RS22710 (HRV88_22645)	4770488..4770775	-	288	WP_038635893.1	NadS family protein	-
HRV88_RS22715 (HRV88_22650)	4770776..4771087	-	312	WP_057066653.1	hypothetical protein	-
HRV88_RS22720 (HRV88_22655)	4771308..4772237	+	930	WP_235125944.1	alpha/beta hydrolase	-
HRV88_RS22725 (HRV88_22660)	4772322..4772633	+	312	WP_048215202.1	type II toxin-antitoxin system HigB family toxin	Toxin
HRV88_RS22730 (HRV88_22665)	4772630..4773076	+	447	WP_235125977.1	helix-turn-helix domain-containing protein	Antitoxin
HRV88_RS22735 (HRV88_22670)	4773094..4774035	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
HRV88_RS22740 (HRV88_22675)	4774080..4774517	-	438	WP_003825289.1	D-aminoacyl-tRNA deacylase	-
HRV88_RS22745 (HRV88_22680)	4774514..4775386	-	873	WP_008786998.1	virulence factor BrkB family protein	-
HRV88_RS22750 (HRV88_22685)	4775380..4775979	-	600	WP_008786997.1	glucose-1-phosphatase	-
HRV88_RS22755 (HRV88_22690)	4776070..4776960	-	891	WP_235125979.1	aldose 1-epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12345.28 Da
• Isoelectric Point: 9.5337

>T158761 WP_048215202.1 NZ_CP054129:4772322-4772633 [Citrobacter portucalensis]
VHVISRKPFNEAMLMYPNHQLALADLLNVLEKKTFIQPEEMKQYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRSHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T158761 NZ_CP069809:c1029862-1029584 [Cupriavidus oxalaticus]
ATGATCCAATCGTTCAGATGCTTTGACACCGCTGCGTTGTTCGACGGCCGTTACGTTGCCCGCTTCGCGAGCATTGAGTT
CATTGCCCAGCGCAAGCTCGAGATGCTTGACGACGCCGCCACCCTGGGCGCGTTGCGCTTTCCGCCGGGAAACCGCCTGG
AAAAACTCGGCGGCGATCGCAAGGGACGCTACAGCATCCGCATCAACGGCCAGTGGAGGATCTGCTTCATCTGGACCCAT
GACGGCCCCGCGGCTGTCGAGATCATCGACTATCACTGA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16689.03 Da
• Isoelectric Point: 6.7580

>AT158761 WP_235125977.1 NZ_CP054129:4772630-4773076 [Citrobacter portucalensis]
MRTHRHMDAASAKKIVDTFSDAVKTIPLLGEDRNDTEYRRALALVEYLVDHDDLENPLFELLCARISKYEKHAPEFKVLN
QHLEKTPSGVSVLRTLMDQYSLKAADLANELGSKSNVSNILNGRRALTVSHIKALAERFNLPADAFIE

Nucleotide

• Length: 447 bp

>AT158761 NZ_CP069809:c1029573-1029244 [Cupriavidus oxalaticus]
ATGGCACGCTTTGAAAACGGCATGCGCCCCATCCATCCGGGCGAAATCCTGCGCGAGGAATACCTGGTGCCGCTGAACAT
GAGCGCCAACGCCCTGGCGCTGGCACTGCGCGTGACGCCGGCCCGCATCAGCGAGATCGTGCGCGAGCAGCGCGGCATCA
CGCCCGACACCGCGCTGCGCCTGACGCGCTATTTTGGTGGCGATGCGCGTTCGTGGATGAATATGCAGGTGACCTACGAC
CTCAAGGTGGCGCAGCGGGACCTGGGCGAGCGCATCGCGGCGGAAGTGGTGCCGCGCGCGGGTATCGACCAGGAAATTCC
GGAAAGCTAG

Structures

Toxin

• AlphaFold DB: A0A5B0T5Y1