Detailed information of TA system TA158754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2531186..2531748
• Replicon: chromosome
• Accession: NZ_CP054129
• Organism: Citrobacter portucalensis strain BY9F36

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HRV88_RS12260
• Protein ID: WP_060683726.1
• Coordinates: 2531470..2531748 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A6H3AUF8
• Locus tag: HRV88_RS12255
• Protein ID: WP_003833018.1
• Coordinates: 2531186..2531470 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRV88_RS12240 (HRV88_12215)	2528881..2529765	+	885	WP_003833024.1	formate dehydrogenase N subunit beta	-
HRV88_RS12245 (HRV88_12220)	2529758..2530414	+	657	WP_003020054.1	formate dehydrogenase-N subunit gamma	-
HRV88_RS12250 (HRV88_12225)	2530544..2531146	+	603	WP_060682194.1	inorganic diphosphatase	-
HRV88_RS12255 (HRV88_12230)	2531186..2531470	-	285	WP_003833018.1	HigA family addiction module antitoxin	Antitoxin
HRV88_RS12260 (HRV88_12235)	2531470..2531748	-	279	WP_060683726.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HRV88_RS12265 (HRV88_12240)	2531969..2533684	-	1716	WP_060682195.1	ABC-F family ATP-binding cassette domain-containing protein	-
HRV88_RS12270 (HRV88_12245)	2533994..2535691	-	1698	WP_003833011.1	malate dehydrogenase	-
HRV88_RS12275 (HRV88_12250)	2535871..2536011	-	141	WP_003833009.1	stationary-phase-induced ribosome-associated protein	-
HRV88_RS12280 (HRV88_12255)	2536239..2536670	+	432	WP_003833007.1	peroxiredoxin OsmC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10649.27 Da
• Isoelectric Point: 9.8965

>T158754 WP_060683726.1 NZ_CP054129:c2531748-2531470 [Citrobacter portucalensis]
MIMNFRHKGLRDLFLHGRTSGVIAKHVRRLRHRLAVIDAASKVTDINMPGYKLHPLVGERDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Nucleotide

• Length: 279 bp

>T158754 NZ_CP069799:c2135013-2134651 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10371.10 Da
• Isoelectric Point: 8.8094

>AT158754 WP_003833018.1 NZ_CP054129:c2531470-2531186 [Citrobacter portucalensis]
MKMANHPRPGDIIQEALEELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNTWSLAEAQ
KSVDISRLRKLAVQ

Nucleotide

• Length: 285 bp

>AT158754 NZ_CP069799:c2135180-2135010 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A6H3AUF8