Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 332065..332731 Replicon chromosome
Accession NZ_CP054129
Organism Citrobacter portucalensis strain BY9F36

Toxin (Protein)


Gene name higB Uniprot ID A0A5B0T6K1
Locus tag HRV88_RS01540 Protein ID WP_047408175.1
Coordinates 332414..332731 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A5B0T6F5
Locus tag HRV88_RS01535 Protein ID WP_048236945.1
Coordinates 332065..332361 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRV88_RS01520 (HRV88_01520) 328510..328983 - 474 WP_003023524.1 transcription elongation factor GreB -
HRV88_RS01525 (HRV88_01525) 329209..329928 + 720 WP_001157751.1 two-component system response regulator OmpR -
HRV88_RS01530 (HRV88_01530) 329925..331277 + 1353 WP_003827658.1 two-component system sensor histidine kinase EnvZ -
HRV88_RS01535 (HRV88_01535) 332065..332361 - 297 WP_048236945.1 NadS family protein Antitoxin
HRV88_RS01540 (HRV88_01540) 332414..332731 - 318 WP_047408175.1 toxin HigB-2 Toxin
HRV88_RS01545 (HRV88_01545) 332854..334476 - 1623 WP_003023529.1 phosphoenolpyruvate carboxykinase (ATP) -
HRV88_RS01550 (HRV88_01550) 334855..336573 + 1719 WP_234096669.1 DUF4153 domain-containing protein -
HRV88_RS01555 (HRV88_01555) 336683..337561 - 879 WP_003023531.1 Hsp33 family molecular chaperone HslO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12255.17 Da        Isoelectric Point: 10.0909

>T158741 WP_047408175.1 NZ_CP054129:c332731-332414 [Citrobacter portucalensis]
MFTFIELQGFSKRRPLLLPDDEFRAFQETLIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSNMLI

Download         Length: 318 bp

>T158741 NZ_CP069798:c1095921-1095667 [Paralysiella testudinis]
ATGGATACCATGAGCTACTCTGCTTTTCGCGCCGGATTGGCCGGAGTGCTGGATAAGGTCAACGACAACCACAAGCCGGT
TTTGGTGACCCGCCAAAACGGCAAACCGGCTGTGGTGATGAGTTTGGAAGATTTTCAGTCTTACGAAGAAACCGCCTATC
TTATGGCCAGCCCCCCTAATGCCGAGCGCCTAAACCGGGCAGTGGCGCAGATTCGCGCCGGAGAAACACAACAACACGGG
ATGCTGGAAGAATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11185.95 Da        Isoelectric Point: 7.2052

>AT158741 WP_048236945.1 NZ_CP054129:c332361-332065 [Citrobacter portucalensis]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQRNPFIINQLKAI

Download         Length: 297 bp

>AT158741 NZ_CP069798:c1095670-1095407 [Paralysiella testudinis]
ATGATGTTGGCGTGGGCCGATGATGCTTGGGACGATTATCTGTATTGGCAGCAAACCGACAAACGCATGTTGAAACGCAT
CAATATGCTGCTGAAAGACATTCAGCGCCAGCCATTTAGCGGATTGGGTGATCCTGAACCGCTGAAACACAATTGGTCAG
ATTTCTGGTCACGCCGCATCGACAAAGAGCACCGGCTGGTTTACCGCGTCAGTAATGATATGGTGATGGTGGCACAGTGC
CGTTACCACTATGGCAAGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5B0T6K1

Antitoxin

Source ID Structure
AlphaFold DB A0A5B0T6F5

References