Detailed information of TA system TA158715

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 2526742..2527397
• Replicon: chromosome
• Accession: NZ_CP054109
• Organism: Bordetella pertussis strain NZ21

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: -
• Locus tag: HRJ75_RS12180
• Protein ID: WP_047122780.1
• Coordinates: 2527146..2527397 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: Q7VY40
• Locus tag: HRJ75_RS12175
• Protein ID: WP_003809516.1
• Coordinates: 2526742..2527143 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRJ75_RS12145 (HRJ75_12155)	2521925..2522947	-	1023	WP_003809532.1	amino acid ABC transporter substrate-binding protein	-
HRJ75_RS12150 (HRJ75_12160)	2523084..2524121	-	1038	WP_023853543.1	L-threonylcarbamoyladenylate synthase	-
HRJ75_RS12155 (HRJ75_12165)	2524141..2525331	-	1191	WP_010930406.1	5-(carboxyamino)imidazole ribonucleotide synthase	-
HRJ75_RS12160 (HRJ75_12170)	2525334..2525849	-	516	WP_019247930.1	5-(carboxyamino)imidazole ribonucleotide mutase	-
HRJ75_RS12165 (HRJ75_12175)	2525999..2526478	-	480	WP_010930405.1	disulfide bond formation protein B	-
HRJ75_RS12170 (HRJ75_12180)	2526486..2526722	-	237	WP_010930404.1	DUF5993 family protein	-
HRJ75_RS12175 (HRJ75_12185)	2526742..2527143	-	402	WP_003809516.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
HRJ75_RS12180 (HRJ75_12190)	2527146..2527397	-	252	WP_047122780.1	type II toxin-antitoxin system MqsR family toxin	Toxin
HRJ75_RS12185 (HRJ75_12195)	2527452..2528333	-	882	WP_003809513.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
HRJ75_RS12190 (HRJ75_12200)	2528558..2529004	+	447	WP_003819827.1	GFA family protein	-
HRJ75_RS12195 (HRJ75_12205)	2529089..2530153	-	1065	WP_003809510.1	fructose-bisphosphate aldolase class II	-
HRJ75_RS12200 (HRJ75_12210)	2530290..2530871	-	582	WP_019247931.1	MOSC domain-containing protein	-
HRJ75_RS12205 (HRJ75_12215)	2531106..2532236	+	1131	WP_010930401.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (10-82)

Antitoxin

• Antitoxin MqsA/HigA-2 (1-131)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 8952.52 Da
• Isoelectric Point: 9.7242

>T158715 WP_047122780.1 NZ_CP054109:c2527397-2527146 [Bordetella pertussis]
IGGAATKKPGQKRRAGAAALGLDFSAMVEIVLALTPRDFHKSMTTHADHRIWQDVYCPVTAMGAVYLKLTVLDGVPIVSF
KEL

Nucleotide

• Length: 252 bp

>T158715 NZ_CP069716:30318-30566 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14528.70 Da
• Isoelectric Point: 8.5005

>AT158715 WP_003809516.1 NZ_CP054109:c2527143-2526742 [Bordetella pertussis]
MKCPVCGAAALARDTRDVPYSYKGETITIAAVTGDWCPACGENVLDGAESARVSEAMLAFNRLVNARRVDPGFIVRVRRK
LGLDQREAAALFGGGTNAFSRYENGKTRPPLALVKLLRLLDRHPELLEEVRAD

Nucleotide

• Length: 402 bp

>AT158715 NZ_CP069716:30563-30910 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3LKS6