Detailed information of TA system TA158658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 2529888..2530543
• Replicon: chromosome
• Accession: NZ_CP054065
• Organism: Bordetella pertussis strain NZ66

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: -
• Locus tag: HRK20_RS12195
• Protein ID: WP_047122780.1
• Coordinates: 2530292..2530543 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: Q7VY40
• Locus tag: HRK20_RS12190
• Protein ID: WP_003809516.1
• Coordinates: 2529888..2530289 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRK20_RS12160 (HRK20_12090)	2525071..2526093	-	1023	WP_003809532.1	amino acid ABC transporter substrate-binding protein	-
HRK20_RS12165 (HRK20_12095)	2526230..2527267	-	1038	WP_023853543.1	L-threonylcarbamoyladenylate synthase	-
HRK20_RS12170 (HRK20_12100)	2527287..2528477	-	1191	WP_010930406.1	5-(carboxyamino)imidazole ribonucleotide synthase	-
HRK20_RS12175 (HRK20_12105)	2528480..2528995	-	516	WP_019247930.1	5-(carboxyamino)imidazole ribonucleotide mutase	-
HRK20_RS12180 (HRK20_12110)	2529145..2529624	-	480	WP_010930405.1	disulfide bond formation protein B	-
HRK20_RS12185 (HRK20_12115)	2529632..2529868	-	237	WP_010930404.1	DUF5993 family protein	-
HRK20_RS12190 (HRK20_12120)	2529888..2530289	-	402	WP_003809516.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
HRK20_RS12195 (HRK20_12125)	2530292..2530543	-	252	WP_047122780.1	type II toxin-antitoxin system MqsR family toxin	Toxin
HRK20_RS12200 (HRK20_12130)	2530598..2531479	-	882	WP_003809513.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
HRK20_RS12205 (HRK20_12135)	2531704..2532150	+	447	WP_003819827.1	GFA family protein	-
HRK20_RS12210 (HRK20_12140)	2532235..2533299	-	1065	WP_003809510.1	fructose-bisphosphate aldolase class II	-
HRK20_RS12215 (HRK20_12145)	2533436..2534017	-	582	WP_019247931.1	MOSC domain-containing protein	-
HRK20_RS12220 (HRK20_12150)	2534252..2535382	+	1131	WP_010930401.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (10-82)

Antitoxin

• Antitoxin MqsA/HigA-2 (1-131)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 8952.52 Da
• Isoelectric Point: 9.7242

>T158658 WP_047122780.1 NZ_CP054065:c2530543-2530292 [Bordetella pertussis]
IGGAATKKPGQKRRAGAAALGLDFSAMVEIVLALTPRDFHKSMTTHADHRIWQDVYCPVTAMGAVYLKLTVLDGVPIVSF
KEL

Nucleotide

• Length: 252 bp

>T158658 NZ_CP069709:967298-967633 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14528.70 Da
• Isoelectric Point: 8.5005

>AT158658 WP_003809516.1 NZ_CP054065:c2530289-2529888 [Bordetella pertussis]
MKCPVCGAAALARDTRDVPYSYKGETITIAAVTGDWCPACGENVLDGAESARVSEAMLAFNRLVNARRVDPGFIVRVRRK
LGLDQREAAALFGGGTNAFSRYENGKTRPPLALVKLLRLLDRHPELLEEVRAD

Nucleotide

• Length: 402 bp

>AT158658 NZ_CP069709:967050-967298 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3LKS6