Detailed information of TA system TA158633

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1983336..1983987
• Replicon: chromosome
• Accession: NZ_CP054060
• Organism: Edwardsiella ictaluri strain T1-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: F8539_RS09410
• Protein ID: WP_198077054.1
• Coordinates: 1983637..1983987 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2A7U2K2
• Locus tag: F8539_RS09405
• Protein ID: WP_049640646.1
• Coordinates: 1983336..1983635 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F8539_RS09375	1978913..1980253	-	1341	WP_049640648.1	Fic family protein	-
F8539_RS09380	1980382..1981152	-	771	WP_198077052.1	Rha family transcriptional regulator	-
F8539_RS09385	1981607..1981936	+	330	WP_015871332.1	type II toxin-antitoxin system RelE/ParE family toxin	-
F8539_RS09390	1981926..1982234	+	309	WP_015871333.1	helix-turn-helix transcriptional regulator	-
F8539_RS09395	1982485..1982742	+	258	WP_198077053.1	DUF4160 domain-containing protein	-
F8539_RS09400	1982752..1983264	+	513	WP_015871334.1	DUF2442 domain-containing protein	-
F8539_RS09405	1983336..1983635	-	300	WP_049640646.1	XRE family transcriptional regulator	Antitoxin
F8539_RS09410	1983637..1983987	-	351	WP_198077054.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F8539_RS09415	1984037..1984348	-	312	WP_049640644.1	DUF1364 domain-containing protein	-
F8539_RS09420	1984351..1984995	-	645	WP_198077055.1	DUF1367 family protein	-
F8539_RS09425	1985105..1985452	-	348	WP_015871339.1	hypothetical protein	-
F8539_RS09430	1985482..1986864	-	1383	WP_049640642.1	replicative DNA helicase	-
F8539_RS09435	1986861..1987811	-	951	WP_198077056.1	helix-turn-helix domain-containing protein	-
F8539_RS09440	1987815..1988003	-	189	WP_198077057.1	hypothetical protein	-
F8539_RS09445	1988013..1988273	-	261	WP_015871343.1	hypothetical protein	-
F8539_RS09450	1988305..1988535	-	231	WP_198077058.1	porin	-
F8539_RS09455	1988532..1988888	-	357	WP_198077059.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1936155..2004122	67967

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12907.85 Da
• Isoelectric Point: 6.9832

>T158633 WP_198077054.1 NZ_CP054060:c1983987-1983637 [Edwardsiella ictaluri]
VWIVETTAAFDEWFTAQSEALQDEMLAALTVLSEFGPNLGRPIVDTLKGAKLANLKELRVQFAGNPIRAFFAFDPERKAI
VLCAGDKTGINEKRFYNSMIKLAEAEFSSHLKKRGS

Nucleotide

• Length: 351 bp

>T158633 NZ_CP069706:c4102471-4101524 [Escherichia coli]
ATGGATGATTTAACCACTCTTTTTTCTGAAGTGGCTGACGCGCTTGGTATTGAGTCTGTCGCAATTGTGGAAAAAGATCA
CTATATCGTTGAGCTTTTGCAATTGCTTAAACCTCTGGTGTTCGATACTCATCAATTGGTGTTTGCCGGAGGAACCGCTC
TGGCAAAATCAAGTGTCGCACTTAATCGCATGTCGGAAGATGTGGATATCAAACTGGTGCCTAAGGATGCATTTCAGGCA
CTTTCCCGCACTCAGCGAAAAAATACCCGTAAAGAAATTGTTCAGGCAGTTTCCAGTGCAATAGCTTCATCGGGTGTGTT
CAATTTTGATGATGATTACCCAAAAGTGACGCGGGATGAATATCGTTATAATGACATACCTGTTCGTTATCCACAGCGAG
TGGTGCAGGCACCTTGTTTGCGTCCTTTTATCCGGCTGGAATTGATGGAAGCGAAGTTACTGGAGCCTTCTGAATTACGG
GATATTCATTCACTGGTAACAGGGATCACGGGAAAAGCGGCTATTGTTTCAGCGTTTCCATGCGCAACTATCATTAGCAC
ACAGGCAGAAAAGCTGGTGGCGATGTTACGTCGTACTGCGGCAGTTGTGCGAGATGTTGAGCGATTTGATGATGCATCGC
TGGTACGTCATATCTACGATACAAACTGTATTGTTGAGACGAGTCATGTTGATATGAGTCAGCTCGTTGATTTTGTTCAA
CAAACTATTGAGCAGGACATTCAGCGTTACGGCAACCAGTACCCGCAGTTTTGCGCGTCTCCAGTTGATGAGCTAAAAAT
GGGACTGGATGAGTTGGCGAACAATCCTCTCTACGAGATGCGCTATCAGCAGTTTGTTTCGCCAATGGTGTATGGAGAGC
GAAAGATCACATGGGATGAGGCTTACTCCAGGTTTAGGAGCTTAGCGCTAGCCATCCTGAATGCTTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11037.74 Da
• Isoelectric Point: 9.9190

>AT158633 WP_049640646.1 NZ_CP054060:c1983635-1983336 [Edwardsiella ictaluri]
MATLDKLLAQRSPESRAKIEARADELRREVLLHQLREELSMSQSELAAAMGVKQPTVARMEQPDNDPRLSTLKRYVTALG
GQISIDVTLPTGKRVGFHL

Nucleotide

• Length: 300 bp

>AT158633 NZ_CP069706:c4102859-4102464 [Escherichia coli]
ATGACGATAAAAGCACGCATTCAGTCACGACTGAAACGTTCTAAACGTTACGTATTCACTCGTGACGACTTTAAAGATAT
TGCAGGCTACGATCAGGTTGGTCGGGTATTGCGCGATCTTGTCAGGGAAGGGCAACTGTTGAAGGTTGGTTATGGCGTGT
ATACCAAAGCACGGCGGAACGGTATTACAGGCAAGGTAATGCCAGCGGCTCCCGGAGGCTCTTCAGCGGTGATTGTCGAA
ACGCTGGAACGCCTGAAAGTACACTATCGTTTTGCTGGCGCAACGCAGGCATACAATAGCGGTACTTCTACGCAGATCCC
AGCTTCACCCGAGATAAAAACCCCCCGGCGTTTTAAACGCGTATTATCTGTAGGGAACAGCAGATTAAATGGATGA

Structures

Antitoxin

• AlphaFold DB: A0A2A7U2K2