Detailed information of TA system TA158584

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 942317..943019
• Replicon: chromosome
• Accession: NZ_CP054049
• Organism: Yersinia kristensenii strain 2012N-4030

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HRD70_RS04055
• Protein ID: WP_050095595.1
• Coordinates: 942317..942631 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HRD70_RS04060
• Protein ID: WP_050095593.1
• Coordinates: 942681..943019 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRD70_RS04045	938150..941197	-	3048	WP_087795298.1	formate dehydrogenase-N subunit alpha	-
HRD70_RS04050	941381..942235	+	855	WP_050095596.1	formate dehydrogenase accessory sulfurtransferase FdhD	-
HRD70_RS04055	942317..942631	+	315	WP_050095595.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HRD70_RS04060	942681..943019	+	339	WP_050095593.1	HigA family addiction module antidote protein	Antitoxin
HRD70_RS04065	943197..943820	+	624	WP_049558554.1	superoxide dismutase [Mn]	-
HRD70_RS04070	944162..946111	+	1950	WP_049558551.1	methyl-accepting chemotaxis protein	-
HRD70_RS04075	946603..946959	-	357	WP_004391136.1	YibL family ribosome-associated protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (7-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-69)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12042.75 Da
• Isoelectric Point: 10.1206

>T158584 WP_050095595.1 NZ_CP054049:942317-942631 [Yersinia kristensenii]
MDKKRSISNFREVWLADFFEHGGKHRKIPSGIENVLARKLDIINAAISYKDLRSPPANHYEELNPPLGGYSSIRVNDQYR
LIFVWAKSKASDLYLDAHGYKKHK

Nucleotide

• Length: 315 bp

>T158584 NZ_CP069692:293145-293420 [Escherichia coli H20]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13144.03 Da
• Isoelectric Point: 5.2065

>AT158584 WP_050095593.1 NZ_CP054049:942681-943019 [Yersinia kristensenii]
MVQAQRKPTSVGDVLLYEYIEPTGIKINDLAEMLQVHRNTISALINNNRKLTTDMAFRLAKAFDTSEEFWLNLQTNVDIW
EVQNDNRLQEEISRIITLDDFLKQKEKYKKLA

Nucleotide

• Length: 339 bp

>AT158584 NZ_CP069692:293417-293776 [Escherichia coli H20]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA