Detailed information of TA system TA158565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 23568..24199
• Replicon: plasmid unnamed2
• Accession: NZ_CP054047
• Organism: Yersinia massiliensis strain 2011N-4075

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HRD68_RS00810
• Protein ID: WP_172986770.1
• Coordinates: 23909..24199 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HRD68_RS00805
• Protein ID: WP_049615233.1
• Coordinates: 23568..23897 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRD68_RS00775	18693..19199	-	507	WP_172986820.1	antirestriction protein ArdA	-
HRD68_RS00780	19221..19457	-	237	Protein_23	DUF1472 domain-containing protein	-
HRD68_RS00785	20332..20895	-	564	WP_172986766.1	helix-turn-helix transcriptional regulator	-
HRD68_RS00790	20995..21399	-	405	WP_172986767.1	plasmid partitioning/stability family protein	-
HRD68_RS00795	21406..22383	-	978	WP_172986768.1	StbA family protein	-
HRD68_RS00800	22640..23140	-	501	WP_172986769.1	hypothetical protein	-
HRD68_RS00805	23568..23897	-	330	WP_049615233.1	HigA family addiction module antidote protein	Antitoxin
HRD68_RS00810	23909..24199	-	291	WP_172986770.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HRD68_RS00815	24337..25158	-	822	WP_172986771.1	N-6 DNA methylase	-
HRD68_RS00820	25535..25960	+	426	WP_172986772.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
HRD68_RS00825	25960..27216	+	1257	WP_120806773.1	translesion error-prone DNA polymerase V subunit UmuC	-
HRD68_RS00830	27675..27902	-	228	WP_145544398.1	hypothetical protein	-
HRD68_RS00835	27952..28569	-	618	WP_172986821.1	ParA family protein	-
HRD68_RS00840	28947..29192	-	246	WP_120806775.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..79154	79154

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (7-85)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11537.24 Da
• Isoelectric Point: 9.3579

>T158565 WP_172986770.1 NZ_CP054047:c24199-23909 [Yersinia massiliensis]
MIKSFRDKNLELFYMESKRDRAIPATIERQLAKKLDMLAAAHIERDLFIPTSNYYKRLSGQFDGWSSMRVNIQWRLMFQW
KDGYAESIYLDPHQDT

Nucleotide

• Length: 291 bp

>T158565 NZ_CP069677:c1936837-1936463 [Escherichia coli O126:H45]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12630.54 Da
• Isoelectric Point: 6.2374

>AT158565 WP_049615233.1 NZ_CP054047:c23897-23568 [Yersinia massiliensis]
MLDTTTREPTTVGEILQEEFLTPLAIGQEELADIMGVTRATVNRLCKGRRRLTVEEATLFSELFETTPEFWLNLQAAHDR
WEAREALQKHKRPPMRPIMEMLGRRISHA

Nucleotide

• Length: 330 bp

>AT158565 NZ_CP069677:c1937294-1936926 [Escherichia coli O126:H45]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA