Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 969435..970354 Replicon chromosome
Accession NZ_CP054014
Organism Mycobacterium tuberculosis strain FDAARGOS_756

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag FOC64_RS04770 Protein ID WP_003900449.1
Coordinates 969435..970040 (+) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag FOC64_RS04775 Protein ID WP_003410124.1
Coordinates 970049..970354 (+) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC64_RS04750 966923..967819 + 897 WP_003410136.1 hypothetical protein -
FOC64_RS04755 968026..968262 + 237 WP_003410133.1 hypothetical protein -
FOC64_RS04760 968214..968891 + 678 WP_003905725.1 hypothetical protein -
FOC64_RS04765 969051..969410 + 360 WP_003410131.1 hypothetical protein -
FOC64_RS04770 969435..970040 + 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FOC64_RS04775 970049..970354 + 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
FOC64_RS04780 970439..970738 + 300 WP_003410120.1 hypothetical protein -
FOC64_RS04785 970754..971170 - 417 WP_003410114.1 hypothetical protein -
FOC64_RS04790 971160..971879 - 720 WP_003410108.1 DUF433 domain-containing protein -
FOC64_RS04795 972121..973161 - 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
FOC64_RS04800 973158..973508 - 351 WP_003918359.1 hypothetical protein -
FOC64_RS04805 973586..974847 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
FOC64_RS04810 974852..975127 - 276 WP_016330411.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T158469 WP_003900449.1 NZ_CP054014:969435-970040 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T158469 NZ_CP069642:2864232-2864543 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT158469 WP_003410124.1 NZ_CP054014:970049-970354 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT158469 NZ_CP069642:2864544-2864834 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References