Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4394702..4395372 Replicon chromosome
Accession NZ_CP054013
Organism Mycobacterium tuberculosis strain FDAARGOS_757

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag FOC65_RS20550 Protein ID WP_003899954.1
Coordinates 4394702..4395046 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag FOC65_RS20555 Protein ID WP_003899955.1
Coordinates 4395043..4395372 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC65_RS20520 4389775..4390635 + 861 WP_003416628.1 alpha/beta hydrolase -
FOC65_RS20525 4390730..4391125 + 396 WP_071854247.1 nitroreductase family deazaflavin-dependent oxidoreductase -
FOC65_RS20530 4391365..4391658 + 294 WP_003416635.1 hypothetical protein -
FOC65_RS20535 4391946..4393235 + 1290 WP_003416640.1 ATP-binding protein -
FOC65_RS20540 4393582..4394016 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
FOC65_RS20545 4394019..4394471 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FOC65_RS20550 4394702..4395046 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FOC65_RS20555 4395043..4395372 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
FOC65_RS20560 4395609..4396870 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
FOC65_RS20565 4397092..4398353 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
FOC65_RS20570 4398626..4398973 + 348 WP_003899956.1 DUF2384 domain-containing protein -
FOC65_RS20575 4398970..4399590 + 621 WP_003899957.1 RES family NAD+ phosphorylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T158451 WP_003899954.1 NZ_CP054013:4394702-4395046 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T158451 NZ_CP069639:c3510895-3510743 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT158451 WP_003899955.1 NZ_CP054013:4395043-4395372 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT158451 NZ_CP069639:3510944-3511001 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References