Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3108906..3109825 Replicon chromosome
Accession NZ_CP054013
Organism Mycobacterium tuberculosis strain FDAARGOS_757

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag FOC65_RS14385 Protein ID WP_003900449.1
Coordinates 3109220..3109825 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag FOC65_RS14380 Protein ID WP_003410124.1
Coordinates 3108906..3109211 (-) Length 102 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC65_RS14350 3103917..3105173 - 1257 WP_003410095.1 HNH endonuclease -
FOC65_RS14355 3105527..3106102 + 576 WP_003410100.1 hypothetical protein -
FOC65_RS14360 3106099..3107139 + 1041 WP_003911735.1 ImmA/IrrE family metallo-endopeptidase -
FOC65_RS14365 3107381..3108100 + 720 WP_003410108.1 DUF433 domain-containing protein -
FOC65_RS14370 3108090..3108506 + 417 WP_003410114.1 hypothetical protein -
FOC65_RS14375 3108522..3108821 - 300 WP_003410120.1 hypothetical protein -
FOC65_RS14380 3108906..3109211 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
FOC65_RS14385 3109220..3109825 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FOC65_RS14390 3109850..3110209 - 360 WP_003410131.1 hypothetical protein -
FOC65_RS14395 3110369..3110827 - 459 Protein_2846 hypothetical protein -
FOC65_RS14400 3110794..3112341 - 1548 WP_003916920.1 DEAD/DEAH box helicase -
FOC65_RS14405 3112851..3113849 - 999 WP_003410146.1 cation diffusion facilitator family transporter -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(86-181)

Antitoxin

(32-92)


Sequences


Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T158433 WP_003900449.1 NZ_CP054013:c3109825-3109220 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T158433 NZ_CP069636:4685016-4685396 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCCCGTCGCAGTTAATCAACAGCATCGATATTCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATGACATTACCCTGGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT158433 WP_003410124.1 NZ_CP054013:c3109211-3108906 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT158433 NZ_CP069636:4684595-4684969 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20


Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References