Detailed information of TA system TA158344

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 46149..46800
• Replicon: plasmid unnamed1
• Accession: NZ_CP053948
• Organism: Acinetobacter sp. WY4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9FYW4
• Locus tag: G4D73_RS14865
• Protein ID: WP_000269911.1
• Coordinates: 46444..46800 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G4D73_RS14860
• Protein ID: WP_001140620.1
• Coordinates: 46149..46451 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4D73_RS14830	41535..42673	+	1139	WP_213687789.1	IS3 family transposase	-
G4D73_RS15540	42737..43262	+	526	Protein_52	transposase	-
G4D73_RS14840	43314..44018	-	705	WP_180087571.1	IS6-like element IS1007 family transposase	-
G4D73_RS14845	44089..45369	-	1281	WP_213687790.1	cation transporter	-
G4D73_RS14850	45460..45852	+	393	WP_004733193.1	Cd(II)/Pb(II)-responsive transcriptional regulator	-
G4D73_RS14855	45946..46107	+	162	Protein_56	IS6 family transposase	-
G4D73_RS14860	46149..46451	-	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
G4D73_RS14865	46444..46800	-	357	WP_000269911.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G4D73_RS14870	47364..47699	-	336	WP_129717937.1	hypothetical protein	-
G4D73_RS14875	47746..48138	-	393	WP_213687791.1	hypothetical protein	-
G4D73_RS14880	48222..48515	-	294	WP_159373035.1	type II toxin-antitoxin system RelE/ParE family toxin	-
G4D73_RS14885	48505..48750	-	246	WP_000246755.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
G4D73_RS14890	48909..49337	+	429	WP_000877730.1	hypothetical protein	-
G4D73_RS14895	49456..50790	-	1335	WP_042084965.1	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..165730	165730
	-	inside	IScluster/Tn	-	-	38803..60155	21352

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13600.60 Da
• Isoelectric Point: 7.1651

>T158344 WP_000269911.1 NZ_CP053948:c46800-46444 [Acinetobacter sp. WY4]
MWTVITTDLFNQWLEQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T158344 NZ_CP069624:c4039654-4039436 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT158344 WP_001140620.1 NZ_CP053948:c46451-46149 [Acinetobacter sp. WY4]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT158344 NZ_CP069624:c4040054-4039680 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A6A1RIA3