Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 5027692..5028422 Replicon chromosome
Accession NZ_CP053925
Organism Serratia marcescens strain 12/2010

Toxin (Protein)


Gene name higB Uniprot ID A0A2S4X738
Locus tag HP475_RS24500 Protein ID WP_033636137.1
Coordinates 5027692..5028003 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2V4GBG9
Locus tag HP475_RS24505 Protein ID WP_033636138.1
Coordinates 5028000..5028422 (+) Length 141 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HP475_RS24470 5023003..5023728 + 726 WP_004937040.1 LPS export ABC transporter ATP-binding protein -
HP475_RS24475 5023781..5025214 + 1434 WP_180555012.1 RNA polymerase factor sigma-54 -
HP475_RS24480 5025238..5025525 + 288 WP_004937038.1 ribosome hibernation promoting factor -
HP475_RS24485 5025681..5026142 + 462 WP_016930212.1 PTS IIA-like nitrogen regulatory protein PtsN -
HP475_RS24490 5026272..5027126 + 855 WP_015379177.1 RNase adapter RapZ -
HP475_RS24495 5027123..5027395 + 273 WP_033648996.1 PTS phosphocarrier protein NPr -
HP475_RS24500 5027692..5028003 + 312 WP_033636137.1 type II toxin-antitoxin system HigB family toxin Toxin
HP475_RS24505 5028000..5028422 + 423 WP_033636138.1 helix-turn-helix domain-containing protein Antitoxin
HP475_RS24510 5028427..5030343 - 1917 WP_033648995.1 BglG family transcription antiterminator -
HP475_RS24515 5030395..5031609 - 1215 WP_049234181.1 lactonase family protein -
HP475_RS24520 5031704..5032444 - 741 WP_004937022.1 KDGP aldolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(81-135)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12259.11 Da        Isoelectric Point: 10.0957

>T158305 WP_033636137.1 NZ_CP053925:5027692-5028003 [Serratia marcescens]
MHIISREPFNNATKQYPNEAAALEAIYKALRRGEFRTPDELKALFPSLDRMKYKEKWWVIDVGGNHLRILFFASFETQKV
FIKHIVSHAEYDKLMQHYRRSSS

Download         Length: 312 bp

>T158305 NZ_CP069621:c106344-105022 [Escherichia coli]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGATAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAACGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCAAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACAGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGGCTCACACCATTTTATGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGAATTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGGTCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin


Download         Length: 141 a.a.        Molecular weight: 15676.97 Da        Isoelectric Point: 4.9590

>AT158305 WP_033636138.1 NZ_CP053925:5028000-5028422 [Serratia marcescens]
MITDAIKAADALLRAVPLLQGSSSQQDYREALELVEYLLENDEHHPLIDMLAKKIAEYEDNSAEFTAFNQRIAQLPQGVA
ALRVLMEQHQLSQSDFEHEIGKKSLVSQILSGKRSLTIPHIMALAKRFNLPPALFLPEAD

Download         Length: 423 bp

>AT158305 NZ_CP069621:c106610-106344 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAGTTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTGACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAAGCA
GCAGAACAGCAAGATTTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S4X738

Antitoxin

Source ID Structure
AlphaFold DB A0A2V4GBG9

References