Detailed information of TA system TA158281

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4818611..4819292
• Replicon: chromosome
• Accession: NZ_CP053922
• Organism: Pseudomonas aeruginosa strain YD001

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6AKP0
• Locus tag: HQN50_RS22585
• Protein ID: WP_003111825.1
• Coordinates: 4818927..4819292 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HQN50_RS22580
• Protein ID: WP_003145733.1
• Coordinates: 4818611..4818934 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQN50_RS22555	4814524..4815162	+	639	WP_019681383.1	hypothetical protein	-
HQN50_RS22560	4815405..4815740	+	336	WP_003140884.1	TM2 domain-containing protein	-
HQN50_RS22565	4815914..4816432	+	519	WP_012614469.1	PAAR domain-containing protein	-
HQN50_RS22570	4816429..4817130	+	702	WP_023088513.1	VRR-NUC domain-containing protein	-
HQN50_RS22575	4817147..4818235	+	1089	WP_023088514.1	DUF3396 domain-containing protein	-
HQN50_RS22580	4818611..4818934	-	324	WP_003145733.1	XRE family transcriptional regulator	Antitoxin
HQN50_RS22585	4818927..4819292	-	366	WP_003111825.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HQN50_RS22590	4819583..4819822	-	240	WP_049876884.1	hypothetical protein	-
HQN50_RS22595	4820029..4820301	+	273	WP_003123013.1	hypothetical protein	-
HQN50_RS22600	4820332..4820757	-	426	WP_003158599.1	VOC family protein	-
HQN50_RS22605	4820858..4821742	+	885	WP_003158600.1	LysR family transcriptional regulator	-
HQN50_RS22610	4821715..4822668	-	954	WP_003085661.1	LysR family transcriptional regulator	-
HQN50_RS22615	4822889..4823323	+	435	WP_003116494.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4807194..4821742	14548

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13880.24 Da
• Isoelectric Point: 4.8219

>T158281 WP_003111825.1 NZ_CP053922:c4819292-4818927 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRHGHMRELRTQHGGRPFRTLYAFDPRRSA
ILLIGGDKTGDDRWYELNVPIADRLYDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T158281 NZ_CP069604:c474341-473862 [Dickeya dianthicola]
GTGGGAATAACGGCACCTGAATTATTGCTGCCTCAACATGCGGTTGTGGATTTTCATTGTTCAGAGCCGTCATTGAATGA
ATGGCTGAAACGCAAGGCGTTAAAAAATCAGACGCTGGGCGCTTCACGCACCTTCGTGGTGTGTGAAGCCGGTACACAGC
GTGTGGTGGGGTTCTATGCCCTCGCGTCGGGCAGCATCCAGCGTCAGGTTGCGCCGGGCGCATTCCGGCGCAATATGCCT
GACCCCATTCCTGTTCTGGTGCTCGGTAGATTAGCCGTTGATGAACGTTATCAACGTATGGGGATCGGTGCCGGGCTCCT
GAAAGATGCGGTACTCCGCTCCCGCAATGTAGCGCAGCAGGTAGGCAACAAAGCGTTATTGGTACATGCGTTATCTGATG
AGGCAAAAGCGTTCTACCAATACTGGGGTTTTGTCCCGTCAGAAATACAGGAACACACCTTATTATTATCGTTATGGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11896.73 Da
• Isoelectric Point: 6.4751

>AT158281 WP_003145733.1 NZ_CP053922:c4818934-4818611 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLHVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT158281 NZ_CP069604:c474604-474332 [Dickeya dianthicola]
ATGCGAACAGAGACAAAAGAAACGCCGATTAATATTCGTGCTAAAGCATCACAGCGGGAGCTGATTGATGTCGCCGCTAA
ATTGCTGTCGAAATCAAGAACAGAGTTTATCCTTGATGCTGCCTGCCGTGAGGCTGAGGATGTGTTGTTGGATCAAAGGC
TGTTTCTGGTCAATGATGAACAATATGATGCTTTCATTCAGGTGCTGGAATCACCTGTCACAGATAATCCGCGTATGAAT
GCATTACTGAACAGGAAATCTCCGTGGGAATAA

Structures

Toxin

• AlphaFold DB: V6AKP0