Detailed information of TA system TA158180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1886143..1886779
• Replicon: chromosome
• Accession: NZ_CP053857
• Organism: Rhizobium pusense strain 76

Toxin (Protein)

• Gene name: graT
• Uniprot ID: U4QGN3
• Locus tag: HQN82_RS22170
• Protein ID: WP_022563816.1
• Coordinates: 1886143..1886424 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: HQN82_RS22175
• Protein ID: WP_020009806.1
• Coordinates: 1886480..1886779 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQN82_RS22150	1882176..1883183	+	1008	WP_022563812.1	iron-siderophore ABC transporter substrate-binding protein	-
HQN82_RS22155	1883180..1884223	+	1044	WP_022563813.1	iron ABC transporter permease	-
HQN82_RS22160	1884226..1885260	+	1035	WP_022563814.1	iron chelate uptake ABC transporter family permease subunit	-
HQN82_RS22165	1885257..1886081	+	825	WP_108081299.1	ABC transporter ATP-binding protein	-
HQN82_RS22170	1886143..1886424	+	282	WP_022563816.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HQN82_RS22175	1886480..1886779	+	300	WP_020009806.1	HigA family addiction module antidote protein	Antitoxin
HQN82_RS22180	1886805..1887671	-	867	WP_173615953.1	helix-turn-helix transcriptional regulator	-
HQN82_RS22185	1887805..1889019	+	1215	WP_022563818.1	amino acid ABC transporter substrate-binding protein	-
HQN82_RS22190	1889090..1889962	+	873	WP_045533916.1	branched-chain amino acid ABC transporter permease	-
HQN82_RS22195	1889959..1890867	+	909	WP_022563820.1	branched-chain amino acid ABC transporter permease	-
HQN82_RS22200	1890864..1891592	+	729	WP_022563821.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10548.99 Da
• Isoelectric Point: 9.0298

>T158180 WP_022563816.1 NZ_CP053857:1886143-1886424 [Rhizobium pusense]
MIRSFKNKLTASIDDGSVKKGFPADLVRRAQQLLTILDAATTVEDLRSPPGNRLEKLSGDREGQYSIRLNKQWRICFVWT
QAGPESVEITDYH

Nucleotide

• Length: 282 bp

>T158180 NZ_CP053857:1886143-1886424 [Rhizobium pusense]
ATGATCCGTTCTTTCAAAAACAAGCTGACCGCCTCAATTGACGACGGATCTGTGAAGAAGGGCTTTCCCGCCGATCTGGT
ACGCCGCGCCCAGCAGCTGCTCACGATCCTGGATGCGGCGACCACGGTAGAAGACCTTCGCTCACCGCCCGGCAATCGTT
TGGAAAAGCTGTCCGGCGATCGTGAGGGCCAATATTCGATCCGGCTCAACAAGCAGTGGCGCATCTGCTTCGTCTGGACG
CAAGCAGGGCCGGAAAGCGTGGAAATAACAGACTATCACTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11072.92 Da
• Isoelectric Point: 8.5148

>AT158180 WP_020009806.1 NZ_CP053857:1886480-1886779 [Rhizobium pusense]
MTISKSPPIHPGEILRELYLEPLGMSAYALARHLNIPRTRIERIAGEKLGITADTALRLAKFFKTTPEYWLNLQNAFDIK
AAAVRLEADLANIKELEAA

Nucleotide

• Length: 300 bp

>AT158180 NZ_CP053857:1886480-1886779 [Rhizobium pusense]
ATGACAATCAGTAAATCGCCACCGATCCATCCGGGCGAAATCCTGCGGGAACTCTATCTTGAGCCGCTCGGGATGTCGGC
TTACGCTCTTGCAAGGCACCTCAATATTCCGCGCACCCGCATAGAACGGATTGCGGGAGAGAAACTTGGTATCACCGCCG
ACACCGCCTTGCGCCTTGCAAAGTTTTTCAAGACCACACCGGAATATTGGCTGAACTTGCAGAACGCCTTTGACATTAAG
GCGGCGGCTGTGAGGCTGGAAGCGGACCTTGCCAATATCAAGGAACTGGAAGCGGCCTAA

Structures

Toxin

• AlphaFold DB: U4QGN3