Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3108861..3109363 Replicon chromosome
Accession NZ_CP053855
Organism Escherichia coli strain 258E

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HPY99_RS14630 Protein ID WP_000767829.1
Coordinates 3109109..3109363 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID -
Locus tag HPY99_RS14625 Protein ID WP_001610372.1
Coordinates 3108861..3109112 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPY99_RS14600 (3104039) 3104039..3105106 - 1068 WP_000080141.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
HPY99_RS14605 (3105106) 3105106..3106176 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
HPY99_RS14610 (3106173) 3106173..3107477 - 1305 WP_001367667.1 histidinol dehydrogenase -
HPY99_RS14615 (3107483) 3107483..3108382 - 900 WP_000131792.1 ATP phosphoribosyltransferase -
HPY99_RS14620 (3108528) 3108528..3108578 - 51 WP_001364200.1 his operon leader peptide -
HPY99_RS14625 (3108861) 3108861..3109112 + 252 WP_001610372.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HPY99_RS14630 (3109109) 3109109..3109363 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HPY99_RS14635 (3109446) 3109446..3110270 + 825 WP_000754737.1 SDR family oxidoreductase -
HPY99_RS14640 (3110316) 3110316..3111245 + 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
HPY99_RS14645 (3111460) 3111460..3111522 + 63 WP_010723108.1 membrane protein YoeI -
HPY99_RS14650 (3111512) 3111512..3112870 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -
HPY99_RS14655 (3113050) 3113050..3114108 + 1059 WP_000492330.1 thiosulfate utilization transporter TsuA/YeeE -
HPY99_RS14660 (3114122) 3114122..3114349 + 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T158168 WP_000767829.1 NZ_CP053855:3109109-3109363 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T158168 NZ_CP069589:4234878-4235162 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9289.55 Da        Isoelectric Point: 4.7954

>AT158168 WP_001610372.1 NZ_CP053855:3108861-3109112 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLIDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT158168 NZ_CP069589:4234642-4234875 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure

References