Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1946483..1947282 Replicon chromosome
Accession NZ_CP053855
Organism Escherichia coli strain 258E

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag HPY99_RS09220 Protein ID WP_000347267.1
Coordinates 1946483..1946947 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HPY99_RS09225 Protein ID WP_001307405.1
Coordinates 1946947..1947282 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPY99_RS09190 (1941484) 1941484..1941918 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
HPY99_RS09195 (1941936) 1941936..1942814 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
HPY99_RS09200 (1942804) 1942804..1943583 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
HPY99_RS09205 (1943594) 1943594..1944067 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HPY99_RS09210 (1944090) 1944090..1945370 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
HPY99_RS09215 (1945619) 1945619..1946428 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HPY99_RS09220 (1946483) 1946483..1946947 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HPY99_RS09225 (1946947) 1946947..1947282 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HPY99_RS09230 (1947431) 1947431..1949002 - 1572 WP_001273741.1 galactarate dehydratase -
HPY99_RS09235 (1949377) 1949377..1950711 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
HPY99_RS09240 (1950727) 1950727..1951497 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T158164 WP_000347267.1 NZ_CP053855:c1946947-1946483 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T158164 NZ_CP069589:3944428-3944778 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT158164 WP_001307405.1 NZ_CP053855:c1947282-1946947 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT158164 NZ_CP069589:3944183-3944434 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References