Detailed information of TA system TA158162

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1152493..1153095
• Replicon: chromosome
• Accession: NZ_CP053855
• Organism: Escherichia coli strain 258E

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HPY99_RS05360
• Protein ID: WP_000897305.1
• Coordinates: 1152784..1153095 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HPY99_RS05355
• Protein ID: WP_000356397.1
• Coordinates: 1152493..1152783 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY99_RS05330 (1148419)	1148419..1149321	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HPY99_RS05335 (1149318)	1149318..1149953	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HPY99_RS05340 (1149950)	1149950..1150879	+	930	WP_001749031.1	formate dehydrogenase accessory protein FdhE	-
HPY99_RS05345 (1151209)	1151209..1151451	-	243	WP_001086388.1	protein YiiF	-
HPY99_RS05350 (1151670)	1151670..1151888	-	219	WP_001295676.1	CopG family transcriptional regulator	-
HPY99_RS05355 (1152493)	1152493..1152783	-	291	WP_000356397.1	NadS family protein	Antitoxin
HPY99_RS05360 (1152784)	1152784..1153095	-	312	WP_000897305.1	hypothetical protein	Toxin
HPY99_RS05365 (1153324)	1153324..1154232	+	909	WP_001162704.1	alpha/beta hydrolase	-
HPY99_RS05370 (1154296)	1154296..1155237	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
HPY99_RS05375 (1155282)	1155282..1155719	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
HPY99_RS05380 (1155716)	1155716..1156588	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HPY99_RS05385 (1156582)	1156582..1157181	-	600	WP_124783521.1	glucose-1-phosphatase	-
HPY99_RS05390 (1157280)	1157280..1158065	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T158162 WP_000897305.1 NZ_CP053855:c1153095-1152784 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T158162 NZ_CP069589:3525225-3525536 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT158162 WP_000356397.1 NZ_CP053855:c1152783-1152493 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT158162 NZ_CP069589:3525537-3525827 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7