Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 330810..331351 Replicon chromosome
Accession NZ_CP053855
Organism Escherichia coli strain 258E

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag HPY99_RS01550 Protein ID WP_000615982.1
Coordinates 331073..331351 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HPY99_RS01545 Protein ID WP_000729704.1
Coordinates 330810..331070 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPY99_RS01520 (326593) 326593..327378 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
HPY99_RS01525 (327350) 327350..329062 + 1713 Protein_303 flagellar biosynthesis protein FlhA -
HPY99_RS01530 (329167) 329167..329445 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
HPY99_RS01535 (329438) 329438..329794 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
HPY99_RS01540 (329851) 329851..330624 - 774 WP_000543899.1 C40 family peptidase -
HPY99_RS01545 (330810) 330810..331070 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HPY99_RS01550 (331073) 331073..331351 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HPY99_RS01555 (331507) 331507..332247 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
HPY99_RS01560 (332218) 332218..332985 - 768 WP_000333380.1 class II glutamine amidotransferase -
HPY99_RS01565 (333191) 333191..333769 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T158160 WP_000615982.1 NZ_CP053855:331073-331351 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T158160 NZ_CP069589:c3130275-3130168 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAGCTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT158160 WP_000729704.1 NZ_CP053855:330810-331070 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT158160 NZ_CP069589:3130332-3130389 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References