Detailed information of TA system TA158117

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2632787..2633586
• Replicon: chromosome
• Accession: NZ_CP053851
• Organism: Escherichia coli strain CE1803A

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: HPM97_RS12460
• Protein ID: WP_000347273.1
• Coordinates: 2632787..2633251 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: HPM97_RS12465
• Protein ID: WP_001308975.1
• Coordinates: 2633251..2633586 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPM97_RS12430	2627788..2628222	-	435	WP_000948834.1	PTS sugar transporter subunit IIA	-
HPM97_RS12435	2628240..2629118	-	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
HPM97_RS12440	2629108..2629887	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
HPM97_RS12445	2629898..2630371	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HPM97_RS12450	2630394..2631674	-	1281	WP_000681961.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HPM97_RS12455	2631923..2632732	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HPM97_RS12460	2632787..2633251	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HPM97_RS12465	2633251..2633586	-	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HPM97_RS12470	2633735..2635306	-	1572	WP_001273756.1	galactarate dehydratase	-
HPM97_RS12475	2635681..2637015	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
HPM97_RS12480	2637031..2637801	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2632787..2644067	11280

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T158117 WP_000347273.1 NZ_CP053851:c2633251-2632787 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T158117 NZ_CP069582:c925323-925069 [Staphylococcus carnosus]
ATTTCATTTTCTCCTGAAGTATTTGAAGATTATAAAAATTGGCAAACTCAAGATAAAAAAGTTTGGAAGAAAATCAATCA
ATTGATTAAAAGTATTGATCGTGATGGCATGTTAGATGGAATTGGTAAGCCAGAACGTTTAAAAGGTGGTTTCAGTGGTT
GGTATAGCAGACGTATCACACAAGATCACAGATTAGTATATAAAGTAACAAGTGAATCAATTTTAGCTGCTTCATGCAAG
TATCATTATCAATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT158117 WP_001308975.1 NZ_CP053851:c2633586-2633251 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT158117 NZ_CP069582:c925579-925331 [Staphylococcus carnosus]
ATGGCTGTTGAAACATACTCAAATGCTAGAAAAAACTTTAGAAGTTTAATTAAACAAGTAAATGAAAACAGTGAAGCTAT
TACCATCACAACAAATGAAGAAAACGCCGTTTTAATGTCTGAAACTGATTATAATGCAATAATGGAGACTCTTTACTTAC
AACAAAACCCAGCTAACGCTGCACATCTCAAACGTTCAATTGAACAACTAGAAAAAGGCGATTCTGTTCAGGTAGAAATA
GATGAGTAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08