Detailed information of TA system TA158013

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1143252..1143850
• Replicon: chromosome
• Accession: NZ_CP053813
• Organism: Vibrio cholerae strain W7G

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I1YFL9
• Locus tag: HPY09_RS05365
• Protein ID: WP_001896384.1
• Coordinates: 1143527..1143850 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HPY09_RS05360
• Protein ID: WP_000058216.1
• Coordinates: 1143252..1143530 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY09_RS05345	1140214..1142034	+	1821	WP_032468029.1	conjugative transfer system coupling protein TraD	-
HPY09_RS05350	1142044..1142604	+	561	WP_149519258.1	conjugative transfer protein	-
HPY09_RS05355	1142591..1143226	+	636	WP_001896322.1	DUF4400 domain-containing protein	-
HPY09_RS05360	1143252..1143530	-	279	WP_000058216.1	helix-turn-helix domain-containing protein	Antitoxin
HPY09_RS05365	1143527..1143850	-	324	WP_001896384.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPY09_RS05370	1144031..1144312	+	282	WP_000433891.1	type IV conjugative transfer system protein TraL	-
HPY09_RS05375	1144309..1144935	+	627	WP_001896361.1	sex pilus assembly	-
HPY09_RS05380	1144919..1145815	+	897	WP_032475897.1	type-F conjugative transfer system secretin TraK	-
HPY09_RS05385	1145818..1147068	+	1251	WP_149519259.1	TraB/VirB10 family protein	-
HPY09_RS05390	1147143..1147715	+	573	WP_142610872.1	TraV family lipoprotein	-
HPY09_RS05395	1147712..1148098	+	387	WP_023584042.1	hypothetical protein	-
HPY09_RS05400	1148139..1148645	-	507	WP_032475876.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1128255..1229902	101647

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12281.33 Da
• Isoelectric Point: 9.7658

>T158013 WP_001896384.1 NZ_CP053813:c1143850-1143527 [Vibrio cholerae]
MSWKIDFYDGVEDQILDMPPKIQARMIKLLELMEKHGANLGPPHTESMGDGLFEIRAKAQEGIGRGLFCYLKGKHIYVLH
AFVKKSQKTPKNELNLARDRQKEVQKS

Nucleotide

• Length: 324 bp

>T158013 NZ_CP069542:2809045-2809332 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10517.07 Da
• Isoelectric Point: 9.9393

>AT158013 WP_000058216.1 NZ_CP053813:c1143530-1143252 [Vibrio cholerae]
MSLQKLKQRALANAEVKNEYDKLESEFSFIDQLLTMRTKAGLTQEQVAERMHTQKSNVSRLEKGNANPSWSTLLKYAHAC
GFELTLKPQKMS

Nucleotide

• Length: 279 bp

>AT158013 NZ_CP069542:2808806-2809045 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: A0A244CN87