Detailed information of TA system TA158004

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 446543..447158
• Replicon: chromosome
• Accession: NZ_CP053811
• Organism: Vibrio cholerae strain W6G

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: HPY05_RS15870
• Protein ID: WP_001162670.1
• Coordinates: 446543..446830 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HPY05_RS15875
• Protein ID: WP_001232701.1
• Coordinates: 446841..447158 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY05_RS15830	441620..442258	+	639	WP_002051085.1	HD domain-containing protein	-
HPY05_RS15835	442417..442830	+	414	WP_175247191.1	GNAT family N-acetyltransferase	-
HPY05_RS15840	442941..443303	+	363	WP_162796981.1	DUF4144 domain-containing protein	-
HPY05_RS15845	443447..443917	+	471	WP_175247100.1	hypothetical protein	-
HPY05_RS15850	444233..444388	+	156	WP_071170622.1	DUF645 family protein	-
HPY05_RS15855	444595..445047	+	453	WP_175247192.1	GNAT family N-acetyltransferase	-
HPY05_RS15860	445194..445790	+	597	WP_175247193.1	class I SAM-dependent methyltransferase	-
HPY05_RS15865	446107..446262	+	156	WP_108911962.1	DUF645 family protein	-
HPY05_RS15870	446543..446830	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPY05_RS15875	446841..447158	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
HPY05_RS15880	447285..447797	-	513	WP_175247194.1	hypothetical protein	-
HPY05_RS15885	447836..447952	+	117	Protein_481	DUF3265 domain-containing protein	-
HPY05_RS15890	448138..448320	+	183	WP_175247341.1	DUF645 family protein	-
HPY05_RS15895	448697..448852	+	156	WP_071170622.1	DUF645 family protein	-
HPY05_RS15900	449053..449763	+	711	WP_175247195.1	hypothetical protein	-
HPY05_RS15905	449756..449869	+	114	WP_175247196.1	DUF3265 domain-containing protein	-
HPY05_RS15910	449901..450533	+	633	WP_001894947.1	HNH endonuclease	-
HPY05_RS15915	450653..451108	+	456	WP_001245329.1	GNAT family N-acetyltransferase	-
HPY05_RS15920	451271..451822	+	552	WP_175247197.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	317868..527734	209866

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T158004 WP_001162670.1 NZ_CP053811:446543-446830 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T158004 NZ_CP069541:c3516-3229 [Acinetobacter pittii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCACGATGTCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCGTTGAGGATCTCAATCCAAGACAGACATACCGATGGCGAAGAACGTTGGCAGACCATTGGGA
AAGTAAAAGGCGTACTAATGCTCTTAGTAGCTCACACCATCTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGACAAGTAACTAAAGCAGAGCGAGATAAATATGAGCATGGTTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT158004 WP_001232701.1 NZ_CP053811:446841-447158 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT158004 NZ_CP069541:c3242-2928 [Acinetobacter pittii]
ATGAGCATGGTTAAATACTCACGCAAAGAACTGAATGAAAAATTCAGCGACAAGCAAGATGCTGAAATTGAACGCTTGCT
TGCTAAGGGAACAGTTTCTGACGATCATCTAGATCTATCAGATATGCCTGAAATTACCGACTGGAGTAATGCTGTACGCC
ACAATCAATTCTATCGTCCAGTGAAGCAACAAACATCTATTCGTTTAGATGCTGACGTACTTGCATGGTTCAAAGCACAA
GGCAAAGGTTATCAGACACGTATGAATGAAATCTTACGGGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36