Detailed information of TA system TA157994

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1143247..1143845
• Replicon: chromosome
• Accession: NZ_CP053810
• Organism: Vibrio cholerae strain W6G

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I1YFL9
• Locus tag: HPY05_RS05365
• Protein ID: WP_001896384.1
• Coordinates: 1143522..1143845 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HPY05_RS05360
• Protein ID: WP_000058216.1
• Coordinates: 1143247..1143525 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY05_RS05345	1140209..1142029	+	1821	WP_032468029.1	conjugative transfer system coupling protein TraD	-
HPY05_RS05350	1142039..1142599	+	561	WP_149519258.1	conjugative transfer protein	-
HPY05_RS05355	1142586..1143221	+	636	WP_001896322.1	DUF4400 domain-containing protein	-
HPY05_RS05360	1143247..1143525	-	279	WP_000058216.1	helix-turn-helix domain-containing protein	Antitoxin
HPY05_RS05365	1143522..1143845	-	324	WP_001896384.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPY05_RS05370	1144026..1144307	+	282	WP_000433891.1	type IV conjugative transfer system protein TraL	-
HPY05_RS05375	1144304..1144930	+	627	WP_001896361.1	sex pilus assembly	-
HPY05_RS05380	1144914..1145811	+	898	Protein_1029	type-F conjugative transfer system secretin TraK	-
HPY05_RS05385	1145814..1147064	+	1251	WP_149519259.1	TraB/VirB10 family protein	-
HPY05_RS05390	1147139..1147711	+	573	WP_142610872.1	TraV family lipoprotein	-
HPY05_RS05395	1147708..1148094	+	387	WP_023584042.1	hypothetical protein	-
HPY05_RS05400	1148135..1148641	-	507	WP_032475876.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1128250..1229894	101644

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12281.33 Da
• Isoelectric Point: 9.7658

>T157994 WP_001896384.1 NZ_CP053810:c1143845-1143522 [Vibrio cholerae]
MSWKIDFYDGVEDQILDMPPKIQARMIKLLELMEKHGANLGPPHTESMGDGLFEIRAKAQEGIGRGLFCYLKGKHIYVLH
AFVKKSQKTPKNELNLARDRQKEVQKS

Nucleotide

• Length: 324 bp

>T157994 NZ_CP069530:27462-27737 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTAATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAATGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AATTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10517.07 Da
• Isoelectric Point: 9.9393

>AT157994 WP_000058216.1 NZ_CP053810:c1143525-1143247 [Vibrio cholerae]
MSLQKLKQRALANAEVKNEYDKLESEFSFIDQLLTMRTKAGLTQEQVAERMHTQKSNVSRLEKGNANPSWSTLLKYAHAC
GFELTLKPQKMS

Nucleotide

• Length: 279 bp

>AT157994 NZ_CP069530:27178-27462 [Escherichia coli]
ATACAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCACTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGATTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A244CN87