Detailed information of TA system TA157992

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 516156..516771
• Replicon: chromosome
• Accession: NZ_CP053809
• Organism: Vibrio cholerae strain SP7G

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: HPY08_RS15790
• Protein ID: WP_001162670.1
• Coordinates: 516156..516443 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HPY08_RS15795
• Protein ID: WP_001232701.1
• Coordinates: 516454..516771 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY08_RS15755	511631..511741	+	111	Protein_565	DUF645 family protein	-
HPY08_RS15760	511983..512396	+	414	WP_000049417.1	VOC family protein	-
HPY08_RS15765	512561..512920	+	360	WP_019830329.1	VOC family protein	-
HPY08_RS15770	513114..513827	+	714	WP_000123660.1	Fic family protein	-
HPY08_RS15775	514189..514299	+	111	Protein_569	DUF645 family protein	-
HPY08_RS15780	514503..514841	+	339	WP_046127316.1	hypothetical protein	-
HPY08_RS15785	514882..515841	-	960	WP_001107099.1	IS30 family transposase	-
HPY08_RS15790	516156..516443	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPY08_RS15795	516454..516771	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
HPY08_RS15800	516914..517432	+	519	WP_114708872.1	GNAT family N-acetyltransferase	-
HPY08_RS15805	517611..517781	+	171	WP_001080654.1	hypothetical protein	-
HPY08_RS15810	517781..518182	+	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
HPY08_RS15815	518455..518637	+	183	WP_000093413.1	DUF645 family protein	-
HPY08_RS15820	518831..519301	-	471	WP_000784954.1	RES family NAD+ phosphorylase	-
HPY08_RS15825	519301..519753	-	453	WP_000024295.1	DUF2384 domain-containing protein	-
HPY08_RS15830	519974..520450	+	477	WP_114708866.1	GNAT family N-acetyltransferase	-
HPY08_RS15835	520791..520918	+	128	Protein_581	DUF645 family protein	-
HPY08_RS15840	520905..521339	+	435	WP_001885561.1	IS200/IS605 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	338606..521339	182733
	-	inside	Integron	-	-	339770..520450	180680
	-	flank	IS/Tn	-	-	520791..521339	548

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T157992 WP_001162670.1 NZ_CP053809:516156-516443 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T157992 NZ_CP069529:c49365-49060 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT157992 WP_001232701.1 NZ_CP053809:516454-516771 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT157992 NZ_CP069529:c49585-49367 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36