Detailed information of TA system TA157946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 555658..556273
• Replicon: chromosome
• Accession: NZ_CP053805
• Organism: Vibrio cholerae strain SL6Y

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: HPY06_RS16005
• Protein ID: WP_001162670.1
• Coordinates: 555986..556273 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HPY06_RS16000
• Protein ID: WP_001232701.1
• Coordinates: 555658..555975 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY06_RS15950	550862..551032	-	171	WP_001080654.1	hypothetical protein	-
HPY06_RS15955	551272..551310	-	39	WP_108244118.1	hypothetical protein	-
HPY06_RS15960	551326..551454	-	129	WP_001886422.1	DUF645 family protein	-
HPY06_RS15965	551775..552011	-	237	WP_046126177.1	hypothetical protein	-
HPY06_RS15970	552014..552397	-	384	Protein_559	PhzF family phenazine biosynthesis protein	-
HPY06_RS15975	552894..553217	-	324	WP_080476600.1	DUF3709 domain-containing protein	-
HPY06_RS15980	553413..553700	-	288	WP_000869999.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HPY06_RS15985	553697..553978	-	282	WP_000086649.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
HPY06_RS15990	554177..554917	-	741	WP_042991272.1	PhzF family phenazine biosynthesis protein	-
HPY06_RS15995	555083..555538	-	456	WP_001245329.1	GNAT family N-acetyltransferase	-
HPY06_RS16000	555658..555975	-	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
HPY06_RS16005	555986..556273	-	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPY06_RS16010	556494..557072	-	579	WP_175231584.1	DJ-1/PfpI family protein	-
HPY06_RS16015	557268..557450	-	183	WP_033933234.1	DUF645 family protein	-
HPY06_RS16020	557745..558200	-	456	WP_055033988.1	hypothetical protein	-
HPY06_RS16025	558417..558659	+	243	WP_002044787.1	type II toxin-antitoxin system ParD family antitoxin	-
HPY06_RS16030	558656..558955	+	300	WP_000802131.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HPY06_RS16035	559189..559425	+	237	WP_071179254.1	RNA-binding protein	-
HPY06_RS16040	559625..559861	-	237	WP_175231586.1	hypothetical protein	-
HPY06_RS16045	560200..560442	+	243	WP_001961623.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
HPY06_RS16050	560461..560776	+	316	Protein_575	type II toxin-antitoxin system RelE/ParE family toxin	-
HPY06_RS16055	560934..561197	-	264	WP_000098716.1	Txe/YoeB family addiction module toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	549333..599478	50145
	-	inside	Integron	-	-	549333..598232	48899

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T157946 WP_001162670.1 NZ_CP053805:c556273-555986 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T157946 NZ_CP069522:c757787-757413 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT157946 WP_001232701.1 NZ_CP053805:c555975-555658 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT157946 NZ_CP069522:c758245-757877 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCATGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36