Detailed information of TA system TA157935

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 506289..506904
• Replicon: chromosome
• Accession: NZ_CP053803
• Organism: Vibrio cholerae strain L6G

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: HPY16_RS15810
• Protein ID: WP_001162670.1
• Coordinates: 506289..506576 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HPY16_RS15815
• Protein ID: WP_001232701.1
• Coordinates: 506587..506904 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY16_RS15780	501539..501853	+	315	WP_000071008.1	DNA-binding transcriptional regulator	-
HPY16_RS15785	501850..501960	+	111	WP_076025865.1	DUF3265 domain-containing protein	-
HPY16_RS15790	502307..503050	+	744	WP_069648816.1	hypothetical protein	-
HPY16_RS15795	503034..503462	+	429	WP_000082746.1	hypothetical protein	-
HPY16_RS15800	504156..505073	+	918	WP_032467917.1	alpha/beta hydrolase	-
HPY16_RS15805	505445..505555	+	111	WP_076025930.1	DUF645 family protein	-
HPY16_RS15810	506289..506576	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPY16_RS15815	506587..506904	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
HPY16_RS15820	507110..507547	+	438	WP_000503164.1	IS200/IS605-like element IS1004 family transposase	-
HPY16_RS15825	507707..507877	+	171	WP_001080654.1	hypothetical protein	-
HPY16_RS15830	507877..508278	+	402	WP_134987537.1	type II toxin-antitoxin system death-on-curing family toxin	-
HPY16_RS15835	508551..508733	+	183	WP_000093413.1	DUF645 family protein	-
HPY16_RS15840	508931..509407	+	477	WP_085604293.1	GNAT family N-acetyltransferase	-
HPY16_RS15845	509748..509908	+	161	Protein_615	DUF645 family protein	-
HPY16_RS15850	509862..510278	+	417	WP_134987692.1	IS200/IS605 family transposase	-
HPY16_RS15855	510374..510847	-	474	WP_000034756.1	FKBP-type peptidyl-prolyl cis-trans isomerase	-
HPY16_RS15860	511069..511683	+	615	WP_002054141.1	OmpA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	blaCARB-7	-	309435..510278	200843
	-	inside	Integron	-	-	310548..509407	198859
	-	inside	IScluster/Tn	-	-	507110..510278	3168

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T157935 WP_001162670.1 NZ_CP053803:506289-506576 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T157935 NZ_CP069517:4344289-4344462 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT157935 WP_001232701.1 NZ_CP053803:506587-506904 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT157935 NZ_CP069517:4343965-4344261 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A0F2HI36