Detailed information of TA system TA157892

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 418980..419595
• Replicon: chromosome
• Accession: NZ_CP053799
• Organism: Vibrio cholerae strain SL5Y

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: HPY14_RS15680
• Protein ID: WP_001162670.1
• Coordinates: 418980..419267 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HPY14_RS15685
• Protein ID: WP_001232701.1
• Coordinates: 419278..419595 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY14_RS15630	414124..414579	+	456	WP_001245329.1	GNAT family N-acetyltransferase	-
HPY14_RS15635	414716..415048	+	333	WP_039470247.1	hypothetical protein	-
HPY14_RS15640	415045..415155	+	111	Protein_440	DUF3265 domain-containing protein	-
HPY14_RS15645	415187..415615	+	429	WP_069648560.1	ester cyclase	-
HPY14_RS15650	415845..416270	+	426	WP_094393057.1	hypothetical protein	-
HPY14_RS15655	416401..416718	-	318	WP_000077449.1	CcdB family protein	-
HPY14_RS15660	416718..416963	-	246	WP_001260797.1	type II toxin-antitoxin system CcdA family antitoxin	-
HPY14_RS15665	417162..417515	+	354	WP_095488391.1	hypothetical protein	-
HPY14_RS15670	417668..418132	+	465	WP_094393021.1	ASCH domain-containing protein	-
HPY14_RS15675	418277..418759	+	483	WP_094393022.1	GNAT family N-acetyltransferase	-
HPY14_RS15680	418980..419267	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPY14_RS15685	419278..419595	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
HPY14_RS15690	420152..420265	+	114	Protein_450	DUF645 family protein	-
HPY14_RS15695	420483..421613	+	1131	WP_094393023.1	DUF4238 domain-containing protein	-
HPY14_RS15700	421817..422206	+	390	WP_000491261.1	VOC family protein	-
HPY14_RS15705	422291..422410	+	120	WP_162843095.1	acetyltransferase	-
HPY14_RS15710	422394..422630	-	237	WP_000772130.1	RNA-binding protein	-
HPY14_RS15715	423646..423903	+	258	WP_055031549.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
HPY14_RS15720	423891..424193	+	303	WP_095486992.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	307775..455729	147954
	-	inside	Integron	-	-	308939..456912	147973

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T157892 WP_001162670.1 NZ_CP053799:418980-419267 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T157892 NZ_CP069501:2665756-2665863 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT157892 WP_001232701.1 NZ_CP053799:419278-419595 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT157892 NZ_CP069501:c2665709-2665643 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A0F2HI36