Detailed information of TA system TA157876

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 402434..403049
• Replicon: chromosome
• Accession: NZ_CP053797
• Organism: Vibrio cholerae strain SL4G

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: HPY13_RS15595
• Protein ID: WP_001162670.1
• Coordinates: 402434..402721 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HPY13_RS15600
• Protein ID: WP_001232701.1
• Coordinates: 402732..403049 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY13_RS15545	397578..398033	+	456	WP_001245329.1	GNAT family N-acetyltransferase	-
HPY13_RS15550	398170..398502	+	333	WP_039470247.1	hypothetical protein	-
HPY13_RS15555	398499..398609	+	111	Protein_407	DUF3265 domain-containing protein	-
HPY13_RS15560	398641..399069	+	429	WP_069648560.1	ester cyclase	-
HPY13_RS15565	399299..399724	+	426	WP_094393057.1	hypothetical protein	-
HPY13_RS15570	399855..400172	-	318	WP_000077449.1	CcdB family protein	-
HPY13_RS15575	400172..400417	-	246	WP_001260797.1	type II toxin-antitoxin system CcdA family antitoxin	-
HPY13_RS15580	400616..400969	+	354	WP_095488391.1	hypothetical protein	-
HPY13_RS15585	401122..401586	+	465	WP_094393021.1	ASCH domain-containing protein	-
HPY13_RS15590	401731..402213	+	483	WP_094393022.1	GNAT family N-acetyltransferase	-
HPY13_RS15595	402434..402721	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPY13_RS15600	402732..403049	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
HPY13_RS15605	403606..403719	+	114	Protein_417	DUF645 family protein	-
HPY13_RS15610	403937..405067	+	1131	WP_094393023.1	DUF4238 domain-containing protein	-
HPY13_RS15615	405271..405660	+	390	WP_000491261.1	VOC family protein	-
HPY13_RS15620	405745..405864	+	120	WP_162843095.1	acetyltransferase	-
HPY13_RS15625	405848..406084	-	237	WP_000772130.1	RNA-binding protein	-
HPY13_RS15630	407100..407357	+	258	WP_055031549.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
HPY13_RS15635	407345..407647	+	303	WP_095486992.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	blaCARB-7	-	306379..444087	137708
	-	inside	Integron	-	-	307543..445270	137727

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T157876 WP_001162670.1 NZ_CP053797:402434-402721 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T157876 NZ_CP069500:3696818-3697027 [Escherichia coli]
ATGGTTGATTATCCAAAACGTGATGCTATTTCCAACATCAACGCTATAATTGTAGATACATTTTTGTATATACAATTAGG
GATGGCAACTATGCCAATGGAGTTTGAATGGAATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTG
AAGATGCGGTGTTAGTGCTTGATGATCCACAACGAACGGAGCCGCTATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT157876 WP_001232701.1 NZ_CP053797:402732-403049 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT157876 NZ_CP069500:3697024-3697326 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGTGCTGGCGAA
CAAATTCGACGAGGATATTGATTACAGCGACATCCCATCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36