Detailed information of TA system TA157861

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 357052..357667
• Replicon: chromosome
• Accession: NZ_CP053795
• Organism: Vibrio cholerae strain W10G

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: HPY12_RS15550
• Protein ID: WP_001162670.1
• Coordinates: 357052..357339 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HPY12_RS15555
• Protein ID: WP_001898235.1
• Coordinates: 357350..357667 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY12_RS15510	352590..352772	+	183	WP_019281482.1	DUF645 family protein	-
HPY12_RS15515	352887..352970	+	84	WP_080099571.1	acetyltransferase	-
HPY12_RS15520	352983..353342	+	360	WP_175234259.1	VOC family protein	-
HPY12_RS15525	353639..353818	+	180	WP_080389342.1	DUF645 family protein	-
HPY12_RS15530	354019..354507	+	489	WP_057569088.1	hypothetical protein	-
HPY12_RS15535	355097..355612	+	516	Protein_326	ClbS/DfsB family four-helix bundle protein	-
HPY12_RS15540	355920..356102	+	183	WP_043992545.1	DUF645 family protein	-
HPY12_RS15545	356391..356816	+	426	WP_001898239.1	hypothetical protein	-
HPY12_RS15550	357052..357339	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPY12_RS15555	357350..357667	+	318	WP_001898235.1	HigA family addiction module antidote protein	Antitoxin
HPY12_RS15560	357787..358302	+	516	WP_000090010.1	hypothetical protein	-
HPY12_RS15565	358564..358800	+	237	Protein_332	DUF3709 domain-containing protein	-
HPY12_RS15570	359088..359417	+	330	WP_043990318.1	DHCW motif cupin fold protein	-
HPY12_RS15575	359728..359853	+	126	WP_080285473.1	DUF645 family protein	-
HPY12_RS15580	359869..359907	+	39	WP_148506239.1	hypothetical protein	-
HPY12_RS15585	360092..360382	-	291	WP_000221352.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HPY12_RS15590	360372..360620	-	249	WP_000213183.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
HPY12_RS15595	360862..361185	+	324	WP_080375416.1	DUF3709 domain-containing protein	-
HPY12_RS15600	361459..362463	+	1005	WP_000964934.1	LD-carboxypeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	306166..386842	80676
	-	inside	Integron	-	-	307330..385669	78339

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T157861 WP_001162670.1 NZ_CP053795:357052-357339 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T157861 NZ_CP069499:c115424-115275 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11689.63 Da
• Isoelectric Point: 10.6504

>AT157861 WP_001898235.1 NZ_CP053795:357350-357667 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRFQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT157861 NZ_CP069499:115472-115528 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A0F2HI36