Detailed information of TA system TA157859

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 326080..326695
• Replicon: chromosome
• Accession: NZ_CP053795
• Organism: Vibrio cholerae strain W10G

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: HPY12_RS15290
• Protein ID: WP_001162670.1
• Coordinates: 326080..326367 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HPY12_RS15295
• Protein ID: WP_001232701.1
• Coordinates: 326378..326695 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY12_RS15255	321518..321982	+	465	WP_057568348.1	GNAT family N-acetyltransferase	-
HPY12_RS15260	322102..322497	+	396	WP_000046952.1	hypothetical protein	-
HPY12_RS15265	322710..323099	+	390	WP_055065342.1	VOC family protein	-
HPY12_RS15270	323261..324163	+	903	WP_057568338.1	hypothetical protein	-
HPY12_RS15275	324275..324775	+	501	WP_000686372.1	NAD(P)H-dependent oxidoreductase	-
HPY12_RS15280	324866..325228	+	363	WP_162096957.1	DUF4144 domain-containing protein	-
HPY12_RS15285	325389..325859	+	471	WP_114726119.1	GNAT family N-acetyltransferase	-
HPY12_RS15290	326080..326367	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPY12_RS15295	326378..326695	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
HPY12_RS15300	326857..327096	+	240	WP_032479075.1	hypothetical protein	-
HPY12_RS15305	327379..327540	+	162	Protein_280	DUF3709 domain-containing protein	-
HPY12_RS15310	327558..327782	+	225	WP_080389379.1	DUF3709 domain-containing protein	-
HPY12_RS15315	328075..328629	+	555	WP_057568340.1	hypothetical protein	-
HPY12_RS15320	328626..328736	+	111	WP_080389381.1	DUF3265 domain-containing protein	-
HPY12_RS15325	328774..329097	+	324	WP_175234256.1	hypothetical protein	-
HPY12_RS15330	329408..329587	+	180	WP_057569791.1	DUF645 family protein	-
HPY12_RS15335	329737..330426	+	690	WP_057568343.1	FMN-binding negative transcriptional regulator	-
HPY12_RS15340	330720..330860	+	141	WP_175234257.1	DUF645 family protein	-
HPY12_RS15345	331109..331648	+	540	WP_000884561.1	hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	306166..386842	80676
	-	inside	Integron	-	-	307330..385669	78339

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T157859 WP_001162670.1 NZ_CP053795:326080-326367 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T157859 NZ_CP069499:c59889-59584 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTATCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT157859 WP_001232701.1 NZ_CP053795:326378-326695 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT157859 NZ_CP069499:c60109-59891 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36