Detailed information of TA system TA157856

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1143624..1144222
• Replicon: chromosome
• Accession: NZ_CP053794
• Organism: Vibrio cholerae strain W10G

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I1YFL9
• Locus tag: HPY12_RS05380
• Protein ID: WP_001896384.1
• Coordinates: 1143899..1144222 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HPY12_RS05375
• Protein ID: WP_000058216.1
• Coordinates: 1143624..1143902 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY12_RS05360	1140586..1142406	+	1821	WP_175234157.1	conjugative transfer system coupling protein TraD	-
HPY12_RS05365	1142416..1142976	+	561	WP_001896321.1	hypothetical protein	-
HPY12_RS05370	1142963..1143598	+	636	WP_057567798.1	DUF4400 domain-containing protein	-
HPY12_RS05375	1143624..1143902	-	279	WP_000058216.1	helix-turn-helix domain-containing protein	Antitoxin
HPY12_RS05380	1143899..1144222	-	324	WP_001896384.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPY12_RS05385	1144403..1144684	+	282	WP_000433891.1	type IV conjugative transfer system protein TraL	-
HPY12_RS05390	1144681..1145307	+	627	WP_001896361.1	sex pilus assembly	-
HPY12_RS05395	1145291..1146187	+	897	WP_175234234.1	type-F conjugative transfer system secretin TraK	-
HPY12_RS05400	1146190..1147479	+	1290	WP_001961079.1	TraB/VirB10 family protein	-
HPY12_RS05405	1147554..1148126	+	573	WP_001944091.1	type IV conjugative transfer system lipoprotein TraV	-
HPY12_RS05410	1148123..1148509	+	387	WP_001896311.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	nueA	996513..1207354	210841

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12281.33 Da
• Isoelectric Point: 9.7658

>T157856 WP_001896384.1 NZ_CP053794:c1144222-1143899 [Vibrio cholerae]
MSWKIDFYDGVEDQILDMPPKIQARMIKLLELMEKHGANLGPPHTESMGDGLFEIRAKAQEGIGRGLFCYLKGKHIYVLH
AFVKKSQKTPKNELNLARDRQKEVQKS

Nucleotide

• Length: 324 bp

>T157856 NZ_CP069498:107855-108136 [Acinetobacter pittii]
ATGGCTATCAAACTTCGTAAATGGGATAGTGCTGAGCATCTTAAAACAGAAGAAGATATGCAAGCTTACCTACAAGCCTG
TATCGATGAATCTAATGGTGATGCTGCATTCATTGCTAAAGCTTTAGGTAACATTGCTAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGTCTAGGCCGAGAAAGTCTTTACAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10517.07 Da
• Isoelectric Point: 9.9393

>AT157856 WP_000058216.1 NZ_CP053794:c1143902-1143624 [Vibrio cholerae]
MSLQKLKQRALANAEVKNEYDKLESEFSFIDQLLTMRTKAGLTQEQVAERMHTQKSNVSRLEKGNANPSWSTLLKYAHAC
GFELTLKPQKMS

Nucleotide

• Length: 279 bp

>AT157856 NZ_CP069498:107552-107854 [Acinetobacter pittii]
ATGTACTCAATATATACCACAGAAGCCTTTGATGATTGGTTTACCAAGTTAAAAGATCAACAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGGAACTTTGGAGATACTGAACCAGTCGGTGAAGGTGTTTCTGAGTTACGTTTCT
TCTTTGGACCTGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTCGTTATTCTCTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTACAATTAGCACAAGATTTAGAGGAGGAGCTGTAA

Structures

Toxin

• AlphaFold DB: A0A244CN87