Detailed information of TA system TA157822

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 775088..775782
• Replicon: chromosome
• Accession: NZ_CP053787
• Organism: Escherichia coli isolate J31

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: HQN81_RS03690
• Protein ID: WP_001459456.1
• Coordinates: 775384..775782 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: HQN81_RS03685
• Protein ID: WP_000554757.1
• Coordinates: 775088..775381 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQN81_RS03665	770720..771217	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
HQN81_RS03670	771441..773180	-	1740	WP_001334802.1	flagellar biosynthesis protein FlhA	-
HQN81_RS03675	773125..773910	+	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
HQN81_RS03680	773981..775036	+	1056	WP_001226164.1	DNA polymerase IV	-
HQN81_RS03685	775088..775381	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HQN81_RS03690	775384..775782	+	399	WP_001459456.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HQN81_RS03695	775792..776244	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
HQN81_RS03700	776478..776744	+	267	WP_001321003.1	hypothetical protein	-
HQN81_RS03705	776656..777213	+	558	WP_001353768.1	peptide chain release factor H	-
HQN81_RS03710	777270..778727	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
HQN81_RS03715	778988..779446	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HQN81_RS03720	779538..780782	+	1245	WP_000189541.1	esterase FrsA	-
-	780042..780122	+	81	NuclAT_11	-	-
-	780042..780122	+	81	NuclAT_11	-	-
-	780042..780122	+	81	NuclAT_11	-	-
-	780042..780122	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	756392..794735	38343

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15502.92 Da
• Isoelectric Point: 8.0949

>T157822 WP_001459456.1 NZ_CP053787:775384-775782 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRM
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T157822 NZ_CP069486:1844388-1844525 [Mammaliicoccus vitulinus]
TTGAATAAACATCCTGAAGAAGAAAATGAAATGGTTCAAGACTTAGATGATGTAAAAGAGCTAGGTAAAGAAATGGAACA
AATTTCAGAACAGAACGACGAAGCATATGAAGAAAAGAAAGAAGAACAAGACTCATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT157822 WP_000554757.1 NZ_CP053787:775088-775381 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT157822 NZ_CP069486:1843775-1844374 [Mammaliicoccus vitulinus]
ATGGCTTTAAACTTTAAAGTATTTGAAACAAAAGATATCGCTGCACGTTACGCTGCAGATGTAGTTCGTAAACAATTTAA
TTCAGATAGTACTTCAGTAGTTGGTATTCACTTAGATGCAGAAAACACTTCATTTTTTGATGAATTATTAGATGACGTAA
AGAAAAATCCAGTGAACTTTAGCCAAATTCATGTTTTAGATTTTGATGGTAAAGAAGATTACTACACAGAATTAGGTGTA
CCAAAAAAACAAGTCTTAAAAACTGATGTCGGTAGTGATGTAGAATCATTTATCAAAGATAAAGTGAACACTGATAAAAA
GAAAAATAAATTGATGTTAGATGTCATTACTTTAACTGAAGATAGTAGAACTGGATTGGATTATAATCAAGCGATACATC
CAGCTAGAGAAGTTATTGTACTTGTTACAGGTAAAGAAAAAGCTAAAGCAGTGAGTCAATTATATGAAGAGCCAGCAAAT
GGTTCAGTTGAATCAGCAAAATTAAAACAACATAGAATGGTCACAGTTTTATTAGATAACGAAGCGGCACAAGGATTGCC
AGAAGACGTTAGATCTTACTTCTCAATTCAATTTTCATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6