Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 273190..273687 Replicon chromosome
Accession NZ_CP053787
Organism Escherichia coli isolate J31

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HQN81_RS01280 Protein ID WP_001173343.1
Coordinates 273514..273687 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HQN81_RS01275 Protein ID WP_000398619.1
Coordinates 273190..273486 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HQN81_RS01255 269921..270640 - 720 WP_000611288.1 two-component system response regulator DcuR -
HQN81_RS01260 270637..272268 - 1632 WP_001216477.1 sensor histidine kinase -
HQN81_RS01265 272449..272679 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
HQN81_RS01270 272691..272963 + 273 WP_000405651.1 N-acetyltransferase -
HQN81_RS01275 273190..273486 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HQN81_RS01280 273514..273687 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HQN81_RS01285 273806..275323 - 1518 WP_001295074.1 lysine--tRNA ligase -
HQN81_RS01290 275560..277017 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 273190..282541 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T157820 WP_001173343.1 NZ_CP053787:273514-273687 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T157820 NZ_CP069486:1075688-1076020 [Mammaliicoccus vitulinus]
ATGGTAAAACAGTACGATATTATTAAATTAAATCTAAATCCTGTTCAAGGCAATGAAAAAGGAAATTATAGACCATGTTT
AGTCGTAAATATTACAGAGTTCACGAAACAATCTGGTTTCGCATGGGTTATACCTATTACATCACGTAAACAGTCACGCT
ATCCTACAGATGTTATAGTTGAAACAGAAAAAGGATTGATTAATGGTGTTGTAGATTGTGCTCACATTAGAACAGTTGAT
TTATATACACGTTCCCATCAAAAAGTTGATGTACTAAAAGCAAATAAGATAGAAGAAGTAAAAGATATTCTAACAGGTAT
TATGGATTTGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT157820 WP_000398619.1 NZ_CP053787:273190-273486 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT157820 NZ_CP069486:1075434-1075694 [Mammaliicoccus vitulinus]
ATGAGTGAACCAACCAATAAGGCATATGCTAAAGTATATAAAAGTGGTAATGGCCAGGTTATCAGTATTAAAAAAGATAT
GTTAGAAAAAGCAGGTTTTAAGATAGGGGATGAACTTGTTATGAATGTTAAAGGTAATCAAATTGTATTAGAAAAACCGA
ATACATTCAAAGACCGTTGGAGAAAGTTTATTGAAGATGGTGGAGAATATGAACGTGGTGAATACGATTGGGGAGAATCA
GTGGGGCGTGAAGAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References