Detailed information of TA system TA157799

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1093110..1093837
• Replicon: chromosome
• Accession: NZ_CP053785
• Organism: Escherichia coli isolate 2-101

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: HQN80_RS05285
• Protein ID: WP_000547564.1
• Coordinates: 1093110..1093421 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HQN80_RS05290
• Protein ID: WP_000126294.1
• Coordinates: 1093418..1093837 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQN80_RS05255	1088252..1089961	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
HQN80_RS05260	1089971..1090513	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
HQN80_RS05265	1090513..1091280	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
HQN80_RS05270	1091277..1091687	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
HQN80_RS05275	1091680..1092150	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
HQN80_RS05280	1092175..1092936	+	762	WP_001026446.1	hypothetical protein	-
HQN80_RS05285	1093110..1093421	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
HQN80_RS05290	1093418..1093837	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
HQN80_RS05295	1093951..1095375	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
HQN80_RS05300	1095384..1096841	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
HQN80_RS05305	1097101..1098111	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
HQN80_RS05310	1098260..1098787	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T157799 WP_000547564.1 NZ_CP053785:1093110-1093421 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T157799 NZ_CP069480:c1301980-1301582 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT157799 WP_000126294.1 NZ_CP053785:1093418-1093837 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT157799 NZ_CP069480:c1302276-1301983 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5