Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 632452..633251 Replicon chromosome
Accession NZ_CP053785
Organism Escherichia coli isolate 2-101

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag HQN80_RS03060 Protein ID WP_000347251.1
Coordinates 632452..632916 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HQN80_RS03065 Protein ID WP_001307405.1
Coordinates 632916..633251 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HQN80_RS03030 627453..627887 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
HQN80_RS03035 627905..628783 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HQN80_RS03040 628773..629552 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
HQN80_RS03045 629563..630036 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HQN80_RS03050 630059..631339 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
HQN80_RS03055 631588..632397 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HQN80_RS03060 632452..632916 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HQN80_RS03065 632916..633251 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HQN80_RS03070 633400..634971 - 1572 WP_001273761.1 galactarate dehydratase -
HQN80_RS03075 635346..636680 + 1335 WP_000599652.1 galactarate/glucarate/glycerate transporter GarP -
HQN80_RS03080 636696..637466 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T157795 WP_000347251.1 NZ_CP053785:c632916-632452 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T157795 NZ_CP069480:287227-287334 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT157795 WP_001307405.1 NZ_CP053785:c633251-632916 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT157795 NZ_CP069480:c287174-287113 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References