Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1756071..1756668 Replicon chromosome
Accession NZ_CP053771
Organism Klebsiella pneumoniae strain BP3636

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag HBJ33_RS08480 Protein ID WP_004142563.1
Coordinates 1756351..1756668 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag HBJ33_RS08475 Protein ID WP_004142561.1
Coordinates 1756071..1756358 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HBJ33_RS08445 1752151..1752399 + 249 WP_002893037.1 DUF1158 domain-containing protein -
HBJ33_RS08450 1752417..1752758 - 342 WP_166689814.1 RamA family antibiotic efflux transcriptional regulator -
HBJ33_RS08455 1752789..1753904 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
HBJ33_RS08460 1754084..1754665 + 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
HBJ33_RS08465 1754665..1755033 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
HBJ33_RS08470 1755153..1755803 + 651 Protein_1671 oxygen-insensitive NAD(P)H nitroreductase -
HBJ33_RS08475 1756071..1756358 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
HBJ33_RS08480 1756351..1756668 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HBJ33_RS08485 1756853..1757896 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
HBJ33_RS08490 1758563..1759429 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
HBJ33_RS08495 1759538..1760965 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T157771 WP_004142563.1 NZ_CP053771:c1756668-1756351 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T157771 NZ_CP069459:4162271-4162585 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT157771 WP_004142561.1 NZ_CP053771:c1756358-1756071 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT157771 NZ_CP069459:4162582-4162998 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References