Detailed information of TA system TA157767

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 54108..54377
• Replicon: plasmid pIncFII
• Accession: NZ_CP053768
• Organism: Klebsiella pneumoniae strain BA10835

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HBJ44_RS28755
• Protein ID: WP_001312861.1
• Coordinates: 54219..54377 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	54108..54173 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBJ44_RS28730	49818..50345	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
HBJ44_RS28735	50403..50636	+	234	WP_000006003.1	DUF905 family protein	-
HBJ44_RS28740	50697..52720	+	2024	Protein_62	ParB/RepB/Spo0J family partition protein	-
HBJ44_RS28745	52789..53223	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HBJ44_RS28750	53220..53939	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	53951..54175	+	225	NuclAT_0	-	-
-	53951..54175	+	225	NuclAT_0	-	-
-	53951..54175	+	225	NuclAT_0	-	-
-	53951..54175	+	225	NuclAT_0	-	-
-	54108..54173	+	66	-	-	Antitoxin
HBJ44_RS28755	54219..54377	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
HBJ44_RS28760	54736..56349	-	1614	WP_000080200.1	IS66-like element ISEc23 family transposase	-
HBJ44_RS28765	56380..56730	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
HBJ44_RS28770	56727..57152	-	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
HBJ44_RS28775	57832..58128	+	297	WP_001272251.1	hypothetical protein	-
HBJ44_RS28780	58239..59060	+	822	WP_001234445.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / mph(A) / rmtB / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12	-	1..97846	97846

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T157767 WP_001312861.1 NZ_CP053768:54219-54377 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T157767 NZ_CP069459:2407523-2407801 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT157767 NZ_CP053768:54108-54173 [Klebsiella pneumoniae]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin