157755

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3965178..3965775 Replicon chromosome
Accession NZ_CP053765
Organism Klebsiella pneumoniae strain BA10835

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag HBJ44_RS19225 Protein ID WP_004142563.1
Coordinates 3965458..3965775 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag HBJ44_RS19220 Protein ID WP_004142561.1
Coordinates 3965178..3965465 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HBJ44_RS19190 3961258..3961506 + 249 WP_002893037.1 DUF1158 domain-containing protein -
HBJ44_RS19195 3961524..3961865 - 342 WP_166689814.1 RamA family antibiotic efflux transcriptional regulator -
HBJ44_RS19200 3961896..3963011 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
HBJ44_RS19205 3963191..3963772 + 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
HBJ44_RS19210 3963772..3964140 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
HBJ44_RS19215 3964260..3964910 + 651 Protein_3769 oxygen-insensitive NAD(P)H nitroreductase -
HBJ44_RS19220 3965178..3965465 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
HBJ44_RS19225 3965458..3965775 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HBJ44_RS19230 3965960..3967003 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
HBJ44_RS19235 3967670..3968536 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
HBJ44_RS19240 3968645..3970072 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T157755 WP_004142563.1 NZ_CP053765:c3965775-3965458 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T157755 NZ_CP069455:32092-32397 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT157755 WP_004142561.1 NZ_CP053765:c3965465-3965178 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT157755 NZ_CP069455:31872-32090 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References