Detailed information of TA system TA157735

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 31947..32373
• Replicon: plasmid pMHMC-004
• Accession: NZ_CP053755
• Organism: Shigella sonnei strain 506

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: COO90_RS26885
• Protein ID: WP_001372321.1
• Coordinates: 31947..32072 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	32149..32373 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COO90_RS26850 (27015)	27015..27242	-	228	WP_000589556.1	conjugal transfer relaxosome protein TraY	-
COO90_RS26855 (27362)	27362..28009	-	648	WP_128867105.1	transcriptional regulator TraJ family protein	-
COO90_RS26860 (28201)	28201..28584	-	384	WP_001151529.1	conjugal transfer relaxosome DNA-binding protein TraM	-
COO90_RS26865 (28917)	28917..29507	+	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
COO90_RS26870 (29804)	29804..30625	-	822	WP_001234445.1	DUF932 domain-containing protein	-
COO90_RS26875 (30736)	30736..31032	-	297	WP_001272251.1	hypothetical protein	-
COO90_RS26880 (31332)	31332..31628	+	297	Protein_42	hypothetical protein	-
COO90_RS26885 (31947)	31947..32072	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
COO90_RS26890 (32014)	32014..32163	-	150	Protein_44	plasmid maintenance protein Mok	-
COO90_RS26895 (32185)	32185..32364	+	180	WP_001334662.1	hypothetical protein	-
- (32149)	32149..32373	-	225	NuclAT_0	-	Antitoxin
- (32149)	32149..32373	-	225	NuclAT_0	-	Antitoxin
- (32149)	32149..32373	-	225	NuclAT_0	-	Antitoxin
- (32149)	32149..32373	-	225	NuclAT_0	-	Antitoxin
COO90_RS26900 (32342)	32342..33104	-	763	Protein_46	plasmid SOS inhibition protein A	-
COO90_RS26905 (33101)	33101..33535	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
COO90_RS26910 (33590)	33590..35548	-	1959	WP_112324844.1	ParB/RepB/Spo0J family partition protein	-
COO90_RS26915 (35612)	35612..35845	-	234	WP_000005971.1	DUF905 domain-containing protein	-
COO90_RS26920 (35901)	35901..36422	-	522	WP_033807850.1	single-stranded DNA-binding protein	-
COO90_RS26925 (36721)	36721..37164	+	444	Protein_51	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / mph(A) / sul1 / qacE / aadA5 / blaTEM-1B	-	1..80217	80217

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T157735 WP_001372321.1 NZ_CP053755:c32072-31947 [Shigella sonnei]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T157735 NZ_CP069453:c647236-646829 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT157735 NZ_CP053755:c32373-32149 [Shigella sonnei]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin