Detailed information of TA system TA157725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3965344..3966071
• Replicon: chromosome
• Accession: NZ_CP053751
• Organism: Shigella sonnei strain 506

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: COO90_RS19980
• Protein ID: WP_000547563.1
• Coordinates: 3965760..3966071 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: COO90_RS19975
• Protein ID: WP_000126296.1
• Coordinates: 3965344..3965763 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COO90_RS19955 (3960395)	3960395..3960922	-	528	WP_001078766.1	electron transport protein HydN	-
COO90_RS19960 (3961070)	3961070..3962080	-	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
COO90_RS19965 (3962340)	3962340..3963797	+	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
COO90_RS19970 (3963806)	3963806..3965230	+	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
COO90_RS19975 (3965344)	3965344..3965763	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
COO90_RS19980 (3965760)	3965760..3966071	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
COO90_RS19985 (3966233)	3966233..3967006	-	774	WP_001026444.1	hypothetical protein	-
COO90_RS19990 (3967031)	3967031..3967501	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
COO90_RS19995 (3967494)	3967494..3967904	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
COO90_RS20000 (3967901)	3967901..3968668	-	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
COO90_RS20005 (3968668)	3968668..3969210	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
COO90_RS20010 (3969220)	3969220..3970917	-	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T157725 WP_000547563.1 NZ_CP053751:c3966071-3965760 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T157725 NZ_CP069451:c3195506-3195252 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT157725 WP_000126296.1 NZ_CP053751:c3965763-3965344 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT157725 NZ_CP069451:c3195754-3195503 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22