157704

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3944105..3944814 Replicon chromosome
Accession NZ_CP053749
Organism Mycobacterium avium subsp. paratuberculosis S397

Toxin (Protein)


Gene name HigB2 Uniprot ID X7V9P5
Locus tag MAPS_RS18535 Protein ID WP_003873387.1
Coordinates 3944105..3944509 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID Q73S10
Locus tag MAPS_RS18540 Protein ID WP_003873388.1
Coordinates 3944506..3944814 (+) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAPS_RS18520 3940358..3941533 + 1176 WP_003874378.1 trehalose-phosphatase -
MAPS_RS18525 3941530..3942396 - 867 WP_003874377.1 MaoC family dehydratase -
MAPS_RS18530 3942618..3943838 + 1221 WP_010948702.1 IS110 family transposase -
MAPS_RS18535 3944105..3944509 + 405 WP_003873387.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MAPS_RS18540 3944506..3944814 + 309 WP_003873388.1 helix-turn-helix transcriptional regulator Antitoxin
MAPS_RS18545 3945301..3946827 - 1527 WP_023883800.1 adenylate/guanylate cyclase domain-containing protein -
MAPS_RS18550 3946922..3948538 - 1617 WP_003873390.1 chaperonin GroEL -
MAPS_RS18555 3948627..3948929 - 303 WP_003873391.1 co-chaperone GroES -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3942639..3943838 1199


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-109)

Antitoxin

HigA2-like, helix-turn-helix domain

(31-91)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 15503.82 Da        Isoelectric Point: 10.3815

>T157704 WP_003873387.1 NZ_CP053749:3944105-3944509 [Mycobacterium avium subsp. paratuberculosis S397]
VRKWEVDLTLIEAWMDALDDEEYDNLIAALEQLEEHGPITRRPFVDTLEGSRHPNMKELRPRPTKAGAHIRVLFAFDTRS
RAIMLIAGDKAGNWSKWYAKHIPIADELFDAHQKRLHKAAAKATNRKPRKGKKR

Download         Length: 405 bp

>T157704 NZ_CP069447:c4497919-4497632 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11445.94 Da        Isoelectric Point: 10.0801

>AT157704 WP_003873388.1 NZ_CP053749:3944506-3944814 [Mycobacterium avium subsp. paratuberculosis S397]
MTNLDDMRRRRPGNRTRIDAIKAEMDREVAQYRLRELREAAGYTQTTLAAAIGVGQNRVSQMEHGDLGTSRVDTLRKYVE
ATGGELEVSVKRPDGSRVLLSL

Download         Length: 309 bp

>AT157704 NZ_CP069447:c4497645-4497343 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB X7V9P5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4U5M0

References