Detailed information of TA system TA157696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5853565..5854160
• Replicon: chromosome
• Accession: NZ_CP053747
• Organism: Pseudomonas aeruginosa strain Pae1255-NDM1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: HJ571_RS27420
• Protein ID: WP_003117425.1
• Coordinates: 5853882..5854160 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HJ571_RS27415
• Protein ID: WP_003113527.1
• Coordinates: 5853565..5853870 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HJ571_RS27400 (HJ571_27405)	5850275..5851138	-	864	WP_023098850.1	integrase domain-containing protein	-
HJ571_RS27405 (HJ571_27410)	5851739..5852881	-	1143	WP_023098851.1	STY4528 family pathogenicity island replication protein	-
HJ571_RS27415 (HJ571_27420)	5853565..5853870	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
HJ571_RS27420 (HJ571_27425)	5853882..5854160	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HJ571_RS27425	5854213..5854341	-	129	Protein_5431	integrase	-
HJ571_RS27430 (HJ571_27430)	5854489..5856717	+	2229	WP_003113525.1	TonB-dependent receptor	-
HJ571_RS27435 (HJ571_27435)	5856787..5857434	-	648	WP_003095021.1	carbonate dehydratase	-
HJ571_RS27440 (HJ571_27440)	5857496..5858734	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T157696 WP_003117425.1 NZ_CP053747:c5854160-5853882 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T157696 NZ_CP069447:2530772-2530874 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT157696 WP_003113527.1 NZ_CP053747:c5853870-5853565 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT157696 NZ_CP069447:c2530912-2530644 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Toxin

• AlphaFold DB: A0A241XLJ5