Detailed information of TA system TA157676

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 45524..45793
• Replicon: plasmid pCP8-3-IncFII
• Accession: NZ_CP053737
• Organism: Escherichia coli strain CP8-3_Sichuan

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HP430_RS23085
• Protein ID: WP_001312861.1
• Coordinates: 45635..45793 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	45524..45589 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP430_RS23045	40563..40823	+	261	WP_071600428.1	hypothetical protein	-
HP430_RS23050	40874..41065	+	192	WP_032140903.1	hypothetical protein	-
HP430_RS23055	41293..41820	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
HP430_RS23060	41876..42109	+	234	WP_000006004.1	DUF905 domain-containing protein	-
HP430_RS23065	42168..44136	+	1969	Protein_54	ParB/RepB/Spo0J family partition protein	-
HP430_RS23070	44205..44639	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HP430_RS23075	44636..45355	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	45367..45591	+	225	NuclAT_0	-	-
-	45367..45591	+	225	NuclAT_0	-	-
-	45367..45591	+	225	NuclAT_0	-	-
-	45367..45591	+	225	NuclAT_0	-	-
-	45367..45591	-	225	NuclAT_0	-	-
HP430_RS23080	45376..45555	-	180	WP_001309233.1	hypothetical protein	-
-	45524..45589	+	66	NuclAT_1	-	Antitoxin
HP430_RS23085	45635..45793	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
HP430_RS23090	46289..46482	+	194	Protein_59	hypothetical protein	-
HP430_RS23095	46708..47004	+	297	WP_001272251.1	hypothetical protein	-
HP430_RS23100	47115..47936	+	822	WP_001234445.1	DUF945 domain-containing protein	-
HP430_RS23105	48233..48835	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
HP430_RS23110	49158..49541	+	384	WP_001354030.1	relaxosome protein TraM	-
HP430_RS23115	49735..50406	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
HP430_RS23120	50543..50770	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(3'')-Ia / aac(3)-VIa / floR / tet(M) / qnrS1	htpB	1..87125	87125

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T157676 WP_001312861.1 NZ_CP053737:45635-45793 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T157676 NZ_CP069445:3638171-3638482 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT157676 NZ_CP053737:45524-45589 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin