Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2426235..2426737 Replicon chromosome
Accession NZ_CP053736
Organism Escherichia coli strain CP8-3_Sichuan

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HP430_RS11730 Protein ID WP_000767829.1
Coordinates 2426235..2426489 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HP430_RS11735 Protein ID WP_001259255.1
Coordinates 2426486..2426737 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HP430_RS11700 2421250..2421477 - 228 WP_063116139.1 sulfurtransferase TusA family protein -
HP430_RS11705 2421491..2422549 - 1059 WP_172614289.1 transport protein YeeE -
HP430_RS11710 2422728..2424086 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HP430_RS11715 2424076..2424138 - 63 WP_010723108.1 membrane protein YoeI -
HP430_RS11720 2424353..2425282 - 930 WP_000803351.1 LysR family transcriptional regulator -
HP430_RS11725 2425328..2426152 - 825 WP_000754737.1 SDR family oxidoreductase -
HP430_RS11730 2426235..2426489 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HP430_RS11735 2426486..2426737 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HP430_RS11740 2427020..2427070 + 51 WP_001364200.1 his operon leader peptide -
HP430_RS11745 2427216..2428115 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
HP430_RS11750 2428121..2429419 + 1299 WP_000009595.1 histidinol dehydrogenase -
HP430_RS11755 2429422..2430492 + 1071 WP_001468894.1 histidinol-phosphate transaminase -
HP430_RS11760 2430492..2431559 + 1068 WP_172614290.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T157668 WP_000767829.1 NZ_CP053736:c2426489-2426235 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T157668 NZ_CP069445:c1473165-1472911 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT157668 WP_001259255.1 NZ_CP053736:c2426737-2426486 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT157668 NZ_CP069445:c1473413-1473162 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References