Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 642759..643453 Replicon chromosome
Accession NZ_CP053736
Organism Escherichia coli strain CP8-3_Sichuan

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag HP430_RS03090 Protein ID WP_001263489.1
Coordinates 643055..643453 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag HP430_RS03085 Protein ID WP_000554758.1
Coordinates 642759..643052 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HP430_RS03060 638576..639043 + 468 WP_000725257.1 flagellar basal body-associated FliL family protein -
HP430_RS03065 639063..639779 + 717 WP_000938723.1 FliA/WhiG family RNA polymerase sigma factor -
HP430_RS03070 639792..640655 + 864 WP_001169518.1 flagellar motor stator protein MotA -
HP430_RS03075 640658..641581 + 924 WP_001232546.1 putative lateral flagellar export/assembly protein LafU -
HP430_RS03080 641652..642707 + 1056 WP_001226164.1 DNA polymerase IV -
HP430_RS03085 642759..643052 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
HP430_RS03090 643055..643453 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
HP430_RS03095 643463..643915 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
HP430_RS03100 644221..644487 + 267 WP_001295202.1 hypothetical protein -
HP430_RS03105 644399..644956 + 558 WP_001352051.1 peptide chain release factor H -
HP430_RS03110 645013..646470 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
HP430_RS03115 646731..647189 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 647785..647865 + 81 NuclAT_11 - -
- 647785..647865 + 81 NuclAT_11 - -
- 647785..647865 + 81 NuclAT_11 - -
- 647785..647865 + 81 NuclAT_11 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T157656 WP_001263489.1 NZ_CP053736:643055-643453 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T157656 NZ_CP069441:3604253-3604567 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT157656 WP_000554758.1 NZ_CP053736:642759-643052 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT157656 NZ_CP069441:3604017-3604250 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References