Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 601133..601674 Replicon chromosome
Accession NZ_CP053736
Organism Escherichia coli strain CP8-3_Sichuan

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag HP430_RS02850 Protein ID WP_000615983.1
Coordinates 601133..601411 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag HP430_RS02855 Protein ID WP_000729703.1
Coordinates 601414..601674 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HP430_RS02835 598715..599293 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
HP430_RS02840 599499..600266 + 768 WP_000333380.1 class II glutamine amidotransferase -
HP430_RS02845 600237..600977 - 741 WP_001225679.1 murein L,D-transpeptidase -
HP430_RS02850 601133..601411 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HP430_RS02855 601414..601674 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HP430_RS02860 601884..602633 + 750 WP_000056849.1 C40 family peptidase -
HP430_RS02865 602809..603306 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
HP430_RS02870 603530..605623 - 2094 WP_001140826.1 flagellar biosynthesis protein FlhA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - hcp1/tssD1 / tssA / tssM / rhs/PAAR / gmhA/lpcA 577034..603306 26272


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T157655 WP_000615983.1 NZ_CP053736:c601411-601133 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T157655 NZ_CP069441:c3567307-3567155 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT157655 WP_000729703.1 NZ_CP053736:c601674-601414 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT157655 NZ_CP069441:3567356-3567413 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References