Detailed information of TA system TA157648

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 29616..29886
• Replicon: plasmid pCP55-IncFII
• Accession: NZ_CP053734
• Organism: Escherichia coli strain CP55_Sichuan

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HP431_RS26030
• Protein ID: WP_001312861.1
• Coordinates: 29728..29886 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	29616..29679 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP431_RS25995	24905..25099	+	195	WP_032175643.1	hypothetical protein	-
HP431_RS26000	25327..25854	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
HP431_RS26005	25912..26145	+	234	WP_000006003.1	DUF905 family protein	-
HP431_RS26010	26206..28229	+	2024	Protein_38	ParB/RepB/Spo0J family partition protein	-
HP431_RS26015	28298..28732	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HP431_RS26020	28729..29448	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	29460..29684	+	225	NuclAT_0	-	-
-	29460..29684	+	225	NuclAT_0	-	-
-	29460..29684	+	225	NuclAT_0	-	-
-	29460..29684	+	225	NuclAT_0	-	-
HP431_RS26025	29469..29648	-	180	WP_001309233.1	hypothetical protein	-
-	29616..29679	-	64	-	-	Antitoxin
HP431_RS26030	29728..29886	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
HP431_RS26035	30364..30557	+	194	Protein_43	hypothetical protein	-
HP431_RS26040	30783..31079	+	297	WP_001272251.1	hypothetical protein	-
HP431_RS26045	31190..32011	+	822	WP_032237714.1	DUF945 domain-containing protein	-
HP431_RS26050	32308..32910	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
HP431_RS26055	33233..33616	+	384	WP_001354030.1	relaxosome protein TraM	-
HP431_RS26060	33810..34481	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
HP431_RS26065	34618..34845	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mcr-3.5 / mph(A)	-	1..70770	70770

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T157648 WP_001312861.1 NZ_CP053734:29728-29886 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T157648 NZ_CP069441:1684538-1684915 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCATACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACCGAACAGTAAATAACATTACTCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Nucleotide

• Length: 64 bp

>AT157648 NZ_CP053734:c29679-29616 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin