Detailed information of TA system TA157642

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 127785..128211
• Replicon: plasmid pCP55-IncFIB
• Accession: NZ_CP053732
• Organism: Escherichia coli strain CP55_Sichuan

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HP431_RS24850
• Protein ID: WP_001312861.1
• Coordinates: 127785..127943 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	127987..128211 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP431_RS24815	122846..123073	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
HP431_RS24820	123167..123853	-	687	WP_000332487.1	PAS domain-containing protein	-
HP431_RS24825	124047..124430	-	384	WP_001151564.1	relaxosome protein TraM	-
HP431_RS24830	124716..125363	+	648	WP_122999502.1	transglycosylase SLT domain-containing protein	-
HP431_RS24835	125660..126481	-	822	WP_032237714.1	DUF945 domain-containing protein	-
HP431_RS24840	126592..126888	-	297	WP_001272251.1	hypothetical protein	-
HP431_RS24845	127114..127307	-	194	Protein_130	hypothetical protein	-
HP431_RS24850	127785..127943	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	127987..128211	-	225	NuclAT_0	-	Antitoxin
-	127987..128211	-	225	NuclAT_0	-	Antitoxin
-	127987..128211	-	225	NuclAT_0	-	Antitoxin
-	127987..128211	-	225	NuclAT_0	-	Antitoxin
HP431_RS24855	128023..128202	+	180	WP_001309233.1	hypothetical protein	-
HP431_RS24860	128223..128942	-	720	WP_021526634.1	plasmid SOS inhibition protein A	-
HP431_RS24865	128939..129373	-	435	WP_032238009.1	conjugation system SOS inhibitor PsiB	-
HP431_RS24870	129428..131386	-	1959	WP_032238008.1	ParB/RepB/Spo0J family partition protein	-
HP431_RS24875	131445..131678	-	234	WP_032188375.1	DUF905 domain-containing protein	-
HP431_RS24880	131736..132263	-	528	WP_136747970.1	single-stranded DNA-binding protein	-
HP431_RS24885	132491..132685	-	195	WP_032175643.1	hypothetical protein	-
HP431_RS24890	132736..133014	-	279	WP_072127934.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN	1..156025	156025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T157642 WP_001312861.1 NZ_CP053732:c127943-127785 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T157642 NZ_CP069441:c285709-285607 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 225 bp

>AT157642 NZ_CP053732:c128211-127987 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin